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引用本文:田翠翠,肖邦定.轮叶黑藻(Hydrilla verticillata)根系泌氧对沉积物中典型铁氧化菌和铁还原菌的影响.湖泊科学,2016,28(4):835-842. DOI:10.18307/2016.0417
TIAN Cuicui,XIAO Bangding.Effects of root radial oxygen loss of Hydrilla verticillata on typical iron-oxidizing bacteria and iron-reducing bacteria in sediment. J. Lake Sci.2016,28(4):835-842. DOI:10.18307/2016.0417
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轮叶黑藻(Hydrilla verticillata)根系泌氧对沉积物中典型铁氧化菌和铁还原菌的影响
田翠翠1,2, 肖邦定1,2
1.中国科学院水生生物研究所, 武汉 430072;2.中国科学院藻类生物学重点实验室, 武汉 430072
摘要:
铁作为地壳中丰度最高的氧化还原敏感元素,对湖泊沉积物的氧化还原作用具有重要的指示意义. 水生植物根系泌氧在根际形成微域的氧化圈,根际是氧化、还原同时发生的生物活跃区. 以轮叶黑藻(Hydrilla verticillata)为研究对象,利用微电极和荧光定量PCR探讨根系泌氧作用对沉积物中典型铁氧化菌(嘉利翁氏菌)和典型铁还原菌(地杆菌)的影响. 结果表明,轮叶黑藻生长迅速,通过根系泌氧作用影响沉积物中铁的价态和形态,是根际铁循环的重要参数,并对根际微区微生物有一定的影响. 根系泌氧使根际嘉利翁氏菌和地杆菌数量增加,进一步影响根际微生物铁循环. 实验结果可为微生物对根际铁循环的研究提供一定的理论基础.
关键词:  轮叶黑藻  根系泌氧  嘉利翁氏菌  地杆菌  荧光定量PCR  沉积物
DOI:10.18307/2016.0417
分类号:
基金项目:国家水体污染控制与治理科技重大专项(2013ZX07102005)资助.
Effects of root radial oxygen loss of Hydrilla verticillata on typical iron-oxidizing bacteria and iron-reducing bacteria in sediment
TIAN Cuicui1,2, XIAO Bangding1,2
1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P.R.China;2.Key Laboratory of Algal Biology of Chinese Academy of Sciences, Wuhan 430072, P.R.China
Abstract:
Iron is the most ubiquitously abundant redox active transition metal on earth, which has an important indication on lake sediments.Root radial oxygen loss (ROL) of aquatic plant allows root to create a rhizosphere oxidized zone and the rhizosphere is a biologically active zone in which oxidation and reduction occurred simultaneously. In the present study, microelectrodes and real time qPCR were used to investigate the effects of ROL of Hydrilla verticillata on typical iron-oxidizing bacteria (Gallionella) and iron-reducing bacteria (Geobacter) in sediment. The results showed that H. verticillata grew quickly, and changed valence state and fraction of iron. ROL was an important parameter, which affected the bacterial communities of rhizosphere. The abundances of Gallionella and Geobacter were higher in the rhizosphere, suggesting that the rhizosphere promoted microbial Fe cycling. These results provide insight into the contribution of microorganism in Fe cycling.
Key words:  Hydrilla verticillata  radial oxygen loss  Gallionella  Geobacter  real time qPCR  sediment
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