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引用本文:沈燕,高光,汤祥明,晁建颖.用FISH技术检测太湖梅梁湾水体中细菌优势种群在冬夏两季的变化.湖泊科学,2010,22(1):57-62. DOI:10.18307/2010.0108
SHEN Yan,Gao Guang,Tang Xiangming,Zhao Jianying.Variation of bacterial dominated population abundance in Meiliang Bay, Lake Taihu detected by FISH during summer and winter. J. Lake Sci.2010,22(1):57-62. DOI:10.18307/2010.0108
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用FISH技术检测太湖梅梁湾水体中细菌优势种群在冬夏两季的变化
沈燕1,2, 高光1, 汤祥明1,3, 晁建颖1,3
1.中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008;2.南京农业大学, 南京 210095;3.中国科学院研究生院, 北京100049
摘要:
采用FISH技术对2008年冬、夏两季太湖梅梁湾水体中细菌优势种群的丰度进行了对比分析。实验中,选取荧光素标记的特异寡核苷酸探针,与太湖梅梁湾水体中的四类细菌优势种群α-变形菌(α-proteobacteria),β-变形菌(β-proteobacteria),γ-变形菌(γ-proteobacteria)和拟杆菌(Bacteroidetes)进行原位杂交。结果表明:①所测定的四类细菌优势种群占总细菌的比例在冬季均显著高于夏季(P<0.01);②水体中α-proteobacteria的丰度随TP浓度的增加而显著增加(R2=0.860,P<0.01);③随着Chl.a浓度的增加,水体中细菌的丰度呈上升的趋势,尤其是其中的γ-proteobacteria,其增大趋势更加显著(R2=0.857,P<0.01);④细菌种群的丰度受水体中PO43-浓度的影响,尤其是对Bacteroidetes的影响更加显著(R2=0.733,P<0.05)。
关键词:  太湖  荧光原位杂交  优势细菌  丰度
DOI:10.18307/2010.0108
分类号:
基金项目:中国科学院知识创新工程重要方向性项目(KZCX2-YW-419);国家重点基础研究发展计划项目(2008CB418103);国家自然科学基金项目(40730529)联合资助
Variation of bacterial dominated population abundance in Meiliang Bay, Lake Taihu detected by FISH during summer and winter
SHEN Yan1,2, Gao Guang1, Tang Xiangming1,3, Zhao Jianying1,3
1.State Key Laboratory of Lake Science and Environment,Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences,Nanjing 210008,P.R.China;2.Nanjing Agricultural University,Nanjing 210095,P.R.China;3.Graduate School of Chinese Academy of Sciences,Beijing 100049,P.R.China
Abstract:
FISH was used to analyze the abundance of dominated bacteria population in Meiliang Bay of Lake Taihu in winter andsummer in 2008. The four dominated bacteria population(a-proteobacteria,b-proteobocteria, y-proteobacteriu, Bacteroidetes) wereanalyzed by in situ hybridization with specific fluorescently(FITC) labeled rRNA-targeted oligonucleotide probes in the test. Theresults indicated;①The proportion of each of the four dominated species in the total number of bacteria was all significantly higherin winter than that in summer(P<0.01);②the abundance of a-proteobacteriu rose notably with the increase of TP concentration(R2=0.860, P<0.01);③there was ascending trend in the abundance of bacteria along with the increase of Chl. a concentra-lion,especially that of y-proteobocteria was more remarkable than the others (R2=0.857,P<0.01);④the abundance of bacte-ria population, especially for Bacteroidetes, was also influenced by PO43---concentration(R2=0.733,P<0.05).
Key words:  Lake Taihu  FISH  dominated bacterial  abundance
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