%0 Journal Article %T 用FISH技术检测太湖梅梁湾水体中细菌优势种群在冬夏两季的变化 %T Variation of bacterial dominated population abundance in Meiliang Bay, Lake Taihu detected by FISH during summer and winter %A 沈燕 %A 高光 %A 汤祥明 %A 晁建颖 %A SHEN,Yan %A Gao Guang %A Tang Xiangming %A Zhao Jianying %J 湖泊科学 %J Journal of Lake Sciences %@ 1003-5427 %V 22 %N 1 %D 2010 %P 57-62 %K 太湖;荧光原位杂交;优势细菌;丰度 %K Lake Taihu;FISH;dominated bacterial;abundance %X 采用FISH技术对2008年冬、夏两季太湖梅梁湾水体中细菌优势种群的丰度进行了对比分析。实验中,选取荧光素标记的特异寡核苷酸探针,与太湖梅梁湾水体中的四类细菌优势种群α-变形菌(α-proteobacteria),β-变形菌(β-proteobacteria),γ-变形菌(γ-proteobacteria)和拟杆菌(Bacteroidetes)进行原位杂交。结果表明:①所测定的四类细菌优势种群占总细菌的比例在冬季均显著高于夏季(P<0.01);②水体中α-proteobacteria的丰度随TP浓度的增加而显著增加(R2=0.860,P<0.01);③随着Chl.a浓度的增加,水体中细菌的丰度呈上升的趋势,尤其是其中的γ-proteobacteria,其增大趋势更加显著(R2=0.857,P<0.01);④细菌种群的丰度受水体中PO43-浓度的影响,尤其是对Bacteroidetes的影响更加显著(R2=0.733,P<0.05)。 %X FISH was used to analyze the abundance of dominated bacteria population in Meiliang Bay of Lake Taihu in winter andsummer in 2008. The four dominated bacteria population(a-proteobacteria,b-proteobocteria, y-proteobacteriu, Bacteroidetes) wereanalyzed by in situ hybridization with specific fluorescently(FITC) labeled rRNA-targeted oligonucleotide probes in the test. Theresults indicated;①The proportion of each of the four dominated species in the total number of bacteria was all significantly higherin winter than that in summer(P<0.01);②the abundance of a-proteobacteriu rose notably with the increase of TP concentration(R2=0.860, P<0.01);③there was ascending trend in the abundance of bacteria along with the increase of Chl. a concentra-lion,especially that of y-proteobocteria was more remarkable than the others (R2=0.857,P<0.01);④the abundance of bacte-ria population, especially for Bacteroidetes, was also influenced by PO43---concentration(R2=0.733,P<0.05). %R 10.18307/2010.0108 %U http://www.jlakes.org/ch/reader/view_abstract.aspx %1 JIS Version 3.0.0