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引用本文:王山杉,刘永定,金传荫,李敦海.Zn2+浓度对固氮鱼腥藻(Anabaena azotica Ley)光能转化特性的影响.湖泊科学,2002,14(4):350-356. DOI:10.18307/2002.0409
WANG Shanshan,LIU Yongding,JIN Chuanyin,LI Dunhai.Effects of Zinc Ion on Photosynthetic System of Anabaena azotica Ley. J. Lake Sci.2002,14(4):350-356. DOI:10.18307/2002.0409
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Zn2+浓度对固氮鱼腥藻(Anabaena azotica Ley)光能转化特性的影响
王山杉, 刘永定, 金传荫, 李敦海
中国科学院水生生物研究所, 武汉430072
摘要:
本实验对在不同Zn2+浓度条件下培养的固氮鱼腥藻(Anabaena azotica Ley)的生长、光合放氧速率和叶绿素荧光参数Fv/Fm进行了测定.结果表明,当Zn2+浓度为1.0μmol/L时,其比生长速率(Specific growth rate)最大,光合放氧速率和Fv/Fm值最高.当Zn2+浓度大于等于5.0μmol/L时会抑制A.azotica Ley的生长和光合作用.对在0μmol/L和5.0μmol/L Zn2+浓度下生长的藻细胞藻胆体-类囊体膜复合物吸收光谱的比较和对与5.0μmol/L Zn2+发生反应的藻蓝蛋白溶液的可见光吸收光谱的分析,发现前者624nm处藻胆体的吸收峰和后者620nm处藻蓝蛋白的吸收峰都因Zn2+的作用而下降,推测藻蓝蛋白为Zn2+影响光合作用的位点之一.碳酸酐酶活性的测定表明Zn2+的浓度水平会影响其活性大小,推测是Zn2+影响光合作用的另-途径.
关键词:  固氮鱼腥藻  锌离子  光合作用  藻蓝蛋白  碳酸酐酶
DOI:10.18307/2002.0409
分类号:
基金项目:国家湖泊重点研究课题(K99-05-35-01)资助;中国科学院水生生物研究所创新领域前沿项目(220316)资助
Effects of Zinc Ion on Photosynthetic System of Anabaena azotica Ley
WANG Shanshan, LIU Yongding, JIN Chuanyin, LI Dunhai
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China
Abstract:
It was found that the concentration of Zn2+< at about 1.0μm0l/L was essential to keep optimal growth of Anabaena azotica Ley and also important to keep a quite high value of photosynthetic oxygen evolution rate, chlorophyll fluorescence (Fv/Fm) level and carbonic anhydrase activity, whereas the relative high concentration of Zn2+(≥5.Oμm) would inhibit the growth of A.azotica Ley, decrease its photosynthetic oxygen evolution rate, chlorophyll fluorescence and CA activity.The study of the absorption spectra of the phycobilisome-thy-lakoid membrane complex and the extraction of phycocyanin indicate that Zn2+ may affect the photosynthesis by its effect on the phycobilisome especially the phycocyanin.Since CA is a Zn2+-containing metalloenzyme and may provide a high concentration of CO2 to Rubisco in the process of CO2 fixation, it is suggested that Zn2+ may affect the photosynthesis by acting on CA.
Key words:  Anabaejia azotica Ley  Photosynthesis  Zinc Ion  Phycocyanin  Carbonic Anhydrase
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