%0 Journal Article %T Zn2+浓度对固氮鱼腥藻(Anabaena azotica Ley)光能转化特性的影响 %T Effects of Zinc Ion on Photosynthetic System of Anabaena azotica Ley %A 王山杉 %A 刘永定 %A 金传荫 %A 李敦海 %A WANG,Shanshan %A LIU,Yongding %A JIN,Chuanyin %A LI,Dunhai %J 湖泊科学 %J Journal of Lake Sciences %@ 1003-5427 %V 14 %N 4 %D 2002 %P 350-356 %K 固氮鱼腥藻;锌离子;光合作用;藻蓝蛋白;碳酸酐酶 %K Anabaejia azotica Ley;Photosynthesis;Zinc Ion;Phycocyanin;Carbonic Anhydrase %X 本实验对在不同Zn2+浓度条件下培养的固氮鱼腥藻(Anabaena azotica Ley)的生长、光合放氧速率和叶绿素荧光参数Fv/Fm进行了测定.结果表明,当Zn2+浓度为1.0μmol/L时,其比生长速率(Specific growth rate)最大,光合放氧速率和Fv/Fm值最高.当Zn2+浓度大于等于5.0μmol/L时会抑制A.azotica Ley的生长和光合作用.对在0μmol/L和5.0μmol/L Zn2+浓度下生长的藻细胞藻胆体-类囊体膜复合物吸收光谱的比较和对与5.0μmol/L Zn2+发生反应的藻蓝蛋白溶液的可见光吸收光谱的分析,发现前者624nm处藻胆体的吸收峰和后者620nm处藻蓝蛋白的吸收峰都因Zn2+的作用而下降,推测藻蓝蛋白为Zn2+影响光合作用的位点之一.碳酸酐酶活性的测定表明Zn2+的浓度水平会影响其活性大小,推测是Zn2+影响光合作用的另-途径. %X It was found that the concentration of Zn2+< at about 1.0μm0l/L was essential to keep optimal growth of Anabaena azotica Ley and also important to keep a quite high value of photosynthetic oxygen evolution rate, chlorophyll fluorescence (Fv/Fm) level and carbonic anhydrase activity, whereas the relative high concentration of Zn2+(≥5.Oμm) would inhibit the growth of A.azotica Ley, decrease its photosynthetic oxygen evolution rate, chlorophyll fluorescence and CA activity.The study of the absorption spectra of the phycobilisome-thy-lakoid membrane complex and the extraction of phycocyanin indicate that Zn2+ may affect the photosynthesis by its effect on the phycobilisome especially the phycocyanin.Since CA is a Zn2+-containing metalloenzyme and may provide a high concentration of CO2 to Rubisco in the process of CO2 fixation, it is suggested that Zn2+ may affect the photosynthesis by acting on CA. %R 10.18307/2002.0409 %U http://www.jlakes.org/ch/reader/view_abstract.aspx %1 JIS Version 3.0.0