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引用本文:孙旭,何晓云,杨柳燕.蓝藻水华对羽摇蚊(Chironomus plumosus)幼虫N2O排放通量的影响.湖泊科学,2018,30(4):1019-1026. DOI:10.18307/2018.0415
SUN Xu,HE Xiaoyun,YANG Liuyan.The effect of algal bloom on the emission flux of nitrous oxide from Chironomus plumosus larvae. J. Lake Sci.2018,30(4):1019-1026. DOI:10.18307/2018.0415
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蓝藻水华对羽摇蚊(Chironomus plumosus)幼虫N2O排放通量的影响
孙旭1,2, 何晓云3, 杨柳燕1
1.南京大学环境学院, 南京 210023;2.环境保护部南京环境科学研究所, 南京 210042;3.浙江省环境监测中心, 杭州 310012
摘要:
底栖动物是湖泊生态环境的重要组成部分,在泥水界面氮循环中扮演着重要的作用,是一个潜在的N2O排放源,但其在富营养化湖泊中N2O排放机制还不清楚.通过室内微宇宙实验,采用气相色谱和现代分子生物学相结合的技术,研究蓝藻存在时底栖动物摇蚊幼虫排放N2O特征及其内在生物学机制.结果显示底栖动物羽摇蚊幼虫(Chironomus plumosus)肠道是N2O排放的主要场所,约占活体释放通量的77%.蓝藻存在时羽摇蚊幼虫的N2O排放通量减少60%左右,肠道内细菌多样性明显下降,肠道中β-变形菌相对丰度增加21%,δ-变形菌相对丰度减少62%,从而改变肠道内细菌群落结构;同时肠道内nirS基因多样性显著增加,出现较多的nirS型反硝化菌的表型,硝酸盐的还原作用得到增强.研究结果有助于揭示富营养化湖泊底栖动物摇蚊幼虫的环境效应,丰富湖泊氮素生物地球化学循环的新过程.
关键词:  N2O  排放通量  蓝藻暴发  羽摇蚊幼虫  肠道微生物  底栖动物
DOI:10.18307/2018.0415
分类号:
基金项目:国家科技重大专项课题(2012ZX07101006)和中国博士后面上基金项目(2016M591762)联合资助.
The effect of algal bloom on the emission flux of nitrous oxide from Chironomus plumosus larvae
SUN Xu1,2, HE Xiaoyun3, YANG Liuyan1
1.School of the Environment, Nanjing University, Nanjing 210023, P. R. China;2.Nanjing Institute of Environmental Sciences, Ministry of Environmental Protection, Nanjing 210042, P. R. China;3.Zhejiang Environmental Monitoring Centre, Hangzhou 310012, P. R. China
Abstract:
Zoobenthos is an indispensable constituent of lake eco-environment and plays an important role in nitrogen cycle between sediment and water. Zoobenthos is the potential source of N2O emission. However, it is still need to further study on the N2O emission mechanism of zoobenthos in the region of algal accumulation. Thus, the combining gas chromatography with modern molecular biological techniques was used to analyze the N2O emission flux and intestinal microorganism to extend our knowledge about the microbiological mechanism of N2O emission in eutrophic lake. The results showed that the N2O emission flux of the gut of Chironomus plumosus larvae decreased about 60%, which accounted for 77% of the total emission flux form C. plumosus, indicating that the gut was the major N2O emission site of C. plumosus larvae. The intestinal bacterial diversity of C. plumosus larvae would decrease with the presence of algal bloom, while the bacterial community structure also changed, the relative abundance of β-proteobacteria increased 21%, whereas δ-proteobacteria decreased 62%. The diversity of nirS-type denitrifiers was higher and their sequences were related to different phylotypes to converse more nitrate to nitrite. Hence, algal bloom increased the richness and diversity of intestinal denitrifying bacteria. The data obtained in this study further elucidates that the algal bloom decreased the emissions of N2O from the zoobenthos in freshwater sediment and could be useful to better understand the environmental effects of zoobenthos and the nitrogen cycle in freshwater ecological systems.
Key words:  Nitrous oxide  emission flux  algal bloom  Chironomus plumosus larvae  intestinal microorganism  zoobenthos
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