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引用本文:曹驰程,王友权,章奇,张晖.典型湖泊天然有机质与四环素的相互作用.湖泊科学,2018,30(4):1004-1011. DOI:10.18307/2018.0413
CAO Chicheng,WANG Youquan,ZHANG Qi,ZHANG Hui.Interactions of tetracycline and dissolved organic matter from freshwater lakes. J. Lake Sci.2018,30(4):1004-1011. DOI:10.18307/2018.0413
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典型湖泊天然有机质与四环素的相互作用
曹驰程1, 王友权2, 章奇1, 张晖1
1.东南大学公共卫生学院环境科学工程教育部重点实验室, 南京 210009;2.南京林业大学生物与环境学院, 江苏省环境工程重点实验室, 南京 210037
摘要:
采用荧光滴定法研究四环素(tetracycline,TC)与太湖溶解性有机质(dissolved organic matter,DOM)和玄武湖DOM的相互作用.三维荧光光谱结合平行因子分析显示,2个湖泊的DOM含有3个荧光组分:类富里酸组分C1、类色氨酸组分C2和类络氨酸组分C3.其中C2的荧光强度远高于C1和C3,是DOM的主要荧光组成.3个荧光组分与TC发生了不同程度的静态猝灭,特别当TC浓度为45.5 μmol/L时,类蛋白组分的荧光强度完全被猝灭(100%),并且猝灭作用改变了DOM分子的微环境极性.同步荧光光谱联合二维相关图谱进一步表明类色氨酸组分优先和TC发生猝灭作用,其次为类络氨酸组分和类富里酸组分.Ryan-Weber方程适于拟合DOM与TC的猝灭过程,2个湖泊的DOM中3个荧光组分的络合常数lgK值范围为5.05~5.85,大小顺序为C2 > C3 > C1.因此,类蛋白组分为主的DOM对TC的络合作用大于类腐殖组分为主的DOM,影响抗生素在湖泊水体中的生物有效性和生态毒性.
关键词:  四环素  溶解性有机质  荧光猝灭  平行因子分析  二维相关图谱  太湖  玄武湖
DOI:10.18307/2018.0413
分类号:
基金项目:东南大学通识课程项目(1125000131)和中央高校基本科研业务费资助项目(2242017K40041)联合资助.
Interactions of tetracycline and dissolved organic matter from freshwater lakes
CAO Chicheng1, WANG Youquan2, ZHANG Qi1, ZHANG Hui1
1.Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, P. R. China;2.Key Laboratory of Environmental Engineering of Jiangsu Province, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, P. R. China
Abstract:
Interactions of tetracycline (TC) with dissolved organic matter (DOM) collected from Lake Taihu and Lake Xuanwu were investigated through fluorescence quenching. Three fluorescent DOM components, fulvic-(C1), tryptophan-(C2), and tyrosine-like (C3) component, were identified in DOM via excitation emission matrix spectra with parallel factor analysis (EEM-PARAFAC). Results from fluorescence quenching titration showed that the fluorescence of 3 components was quenched at different degree by TC through static quenching. When the TC concentration increased to 45.5 μmol/L, the whole florescence of C2 and C3 were almost quenched, while 20% of C1 fluorescence was inaccessible. The quenching also induced conformational changes in DOM molecules. Synchronous fluorescence spectra combined with two dimensional correlation spectroscopy (2D-COS) further revealed that the complexation firstly occurred for tryptophan-like substance, followed by tyrosine-and fulvic-like substance. The associated complexing constants (lgK) of 3 components with TC were in the range of 5.05-5.85, followed the order of C2 > C3 > C1. The strong binding ability of protein-like substances confirmed their prominent role in the TC-DOM complexation. These results highlight the significant impact of DOM on the bioavailability and ecotoxicity of antibiotics in freshwater lakes.
Key words:  Tetracycline  dissolved organic matter  fluorescence quenching  2D-COS  PARAFAC  Lake Taihu  Lake Xuanwu
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