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引用本文:刘菲菲,冯慕华,尚丽霞,何延召,李文朝.温度对铜绿微囊藻(Microcystis aeruginosa)和鱼腥藻(Anabaena sp.)生长及胞外有机物产生的影响.湖泊科学,2014,26(5):780-788. DOI:10.18307/2014.0517
LIU Feifei,FENG Muhua,SHANG Lixia,HE Yanzhao,LI Wenchao.Effects of temperature on the growth and generation of extracellular organic matter of Microcystis aeruginosa and Anabaena sp.. J. Lake Sci.2014,26(5):780-788. DOI:10.18307/2014.0517
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温度对铜绿微囊藻(Microcystis aeruginosa)和鱼腥藻(Anabaena sp.)生长及胞外有机物产生的影响
刘菲菲1,2, 冯慕华1, 尚丽霞1,2, 何延召1,2, 李文朝1
1.中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008;2.中国科学院大学, 北京 100049
摘要:
以巢湖优势种铜绿微囊藻(Microcystis aeruginosa)和鱼腥藻(Anabaena sp.)为研究对象,研究不同温度(35、25和10℃)对这两种藻生长特性和胞外有机物产生的影响.结果表明,温度对铜绿微囊藻和鱼腥藻的藻细胞密度、碱性磷酸酶活性和胞外有机物浓度影响显著.25℃是铜绿微囊藻和鱼腥藻最适宜的生长温度,最高细胞密度分别达到3.12×107 cells/ml和2.03×107 cells/ml.不同温度下两种藻的碱性磷酸酶活性特征,证实了高温对鱼腥藻生长的抑制和低温对铜绿微囊藻生长的抑制.胞外有机物释放总量受蓝藻生物量和单位细胞有机物释放速率的影响.铜绿微囊藻的溶解性有机碳和胞外总多糖释放量在25℃最高,最大值分别为49.28和38.46 mg/L;而鱼腥藻在35℃时释放量最高,最大值分别为45.82和40.60 mg/L;10℃条件抑制了两种藻的生长及胞外有机物的释放.鱼腥藻胞外多糖含量在35℃培养条件下最高,而铜绿微囊藻在10℃条件下最高,说明不利的生长条件会促进蓝藻胞外多糖的分泌.三维荧光图谱分析结果表明,铜绿微囊藻和鱼腥藻胞外有机物以类蛋白质和类腐殖酸为主,温度主要影响藻细胞胞外有机物浓度,而对有机物种类组成没有影响.
关键词:  铜绿微囊藻  鱼腥藻  碱性磷酸酶活性  胞外多糖  温度  巢湖
DOI:10.18307/2014.0517
分类号:
基金项目:国家水体污染控制与治理科技重大专项项目(2011ZX07103-004-02,2012ZX07103-005);中国科学院南京地理与湖泊研究所学科交叉与前沿项目(NIGLAS2012135013)联合资助
Effects of temperature on the growth and generation of extracellular organic matter of Microcystis aeruginosa and Anabaena sp.
LIU Feifei1,2, FENG Muhua1, SHANG Lixia1,2, HE Yanzhao1,2, LI Wenchao1
1.State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China;2.University of Chinese Academy of Sciences, Beijing 100049, P. R. China
Abstract:
We investigated the growth characteristics and dynamic variations of extracellular organic matter (EOM) under the temperature of 35, 25 and 10℃ using Microcystis aeruginosa and Anabaena sp., the two dominant species in Lake Chaohu, and the targeting cyanobacteria in the present study. The results showed that culture temperature played an important role in cell density, alkaline phosphatase activity (APA), and the release of EOM. The most adaptive temperature for Microcystis aeruginosa and Anabaena sp. was 25℃, with the maximum cell densities of 3.12×107 and 2.03×107 cells/ml, respectively. The characteristics of APA under different temperature conditions proved that the inhibition effect of high temperature on the growth of Microcystis aeruginosa and that of low temperature on the growth of Anabaena sp. Total amount of EOM was determined by cyanobacterial biomass and the release rate of unicellular EOM. The highest amounts of dissolved organic carbon and extracellular polysaccharide substances (EPS) for Anabaena sp. were 49.28 and 38.46 mg/L at 25℃, and 45.82 and 40.60 mg/L at 35℃, respectively, whereas low temperature of 10℃ restricted the release of EOM of the two studied cyanobacteria. The results of highest amount of unicellular EPS at 35℃ for Anabaena sp. and at 10℃ for Microcystis aeruginosa suggested that adverse growth conditions promoted the generation of EPS. Excitation-Emission Matrice of EOM showed that main EOM of Microcystis aeruginosa and Anabaena sp. were protein-like and humic acid-like organic substances. Furthermore, the concentrations of organic matters rather than the types of organic matters were influenced by temperature.
Key words:  Microcystis aeruginosa  Anabaena sp.  alkaline phosphatase activity  extracellular polysaccharide  temperature  Lake Chaohu
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