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引用本文:吴东艳,周立志.安徽沿江两个富营养化浅水湖泊克氏原螯虾(Procambarus clarkii)遗传毒性比较.湖泊科学,2012,24(1):129-136. DOI:10.18307/2012.0118
WU Dongyan,ZHOU Lizhi.Genetic toxicity of Procambarus clarkii in two shallow eutrophic lakes along the Yangtze River in Anhui Province. J. Lake Sci.2012,24(1):129-136. DOI:10.18307/2012.0118
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安徽沿江两个富营养化浅水湖泊克氏原螯虾(Procambarus clarkii)遗传毒性比较
吴东艳, 周立志
安徽大学资源与环境工程学院, 安徽大学生物多样性与湿地生态研究所, 合肥 230601
摘要:
富营养化湖泊的蓝藻水华加剧了水体污染,影响湖泊生态系统的稳定.本研究针对安徽沿江富营养化程度不同的巢湖和菜子湖,2010年4 9月份,利用微核和彗星电泳实验检测水体污染物对克氏原螯虾(Procambarus clarkii)血细胞的微核率和DNA损伤,评价湖泊污染物的遗传毒性效应.巢湖和菜子湖细胞微核率最低值分别为0.316±0.131和0.243±0.063,最高值分别为0.624±0.136和0.360±0.081;巢湖DNA损伤三项指标TailDNA、TM和OTM最高值为7.59±1.58、2.75±1.55和3.12±1.45,菜子湖为5.02±1.42、1.07±0.16和1.19±0.26.两个湖泊克氏原螯虾血细胞微核率和DNA损伤都表现为随时间推移而增加,且巢湖6、7月份血细胞微核率和DNA损伤增加幅度最大.7、8、9三个月份,两湖泊细胞微核率和DNA损伤差异性达到极显著.彗星实验和微核实验结果一致,但前者更敏感.两种不同的遗传毒性检测方法结合能更好地检测污染物对机体产生的遗传毒性作用.
关键词:  遗传毒性  微核实验  彗星实验  巢湖  富营养化湖泊  克氏原螯虾  菜子湖
DOI:10.18307/2012.0118
分类号:
基金项目:安徽省教育厅重点科研项目(KJ2009A179);安徽省自然科学基金项目(11040606M76);安徽大学人才培养项目联合资助
Genetic toxicity of Procambarus clarkii in two shallow eutrophic lakes along the Yangtze River in Anhui Province
WU Dongyan, ZHOU Lizhi
School of Resources and Environmental Engineering, Institute of Biodiversity and Wetland Ecology, Anhui University, Hefei 230601, P. R. China
Abstract:
Cyanobacterial bloom promotes pollution of the eutrophic lakes and affects the stability of lake ecosystems. In the present research we detected DNA damages and micronucleus blood cells of Procambarus clarkii in Lake Caizi and Lake Chaohu, two different eutrophic lakes along the Yangtze River in Anhui Province by micronucleus test and comet assay to assess the genetic toxicity effects on the blood cells during the period from April to September, 2010. The lowest micronucleus ratios were 0.316±0.131 and 0.243±0.063, and the highest values were 0.624±0.136 and 0.360±0.081 in Lake Chaohu and Lake Caizi, respectively. The highest values of the three indicators of DNA damage ratios, TailDNA, TM and OTM, which occurred in Lake Chaohu, were 7.59±1.58, 2.75±1.55, 3.12±1.45, respectively, and 5.02±1.42, 1.07±0.16, 1.19±0.26, respectively in Lake Caizi. DNA damage and micronucleus ratios increased with time in the two lakes, and in Lake Chaohu during the period from June to July by large amplitude. The DNA damage and micronucleus reached significant differences between the two lakes in July, August and September. Detection results were the same for the two different tests, but comet assay was more sensitive then micronucleus test. So we suggest that it's necessary to combine the two tests to get more effective detection of genetic toxicity.
Key words:  Genetic toxicity  micronucleus test  comet assay  Lake Chaohu  eutrophic lake  Procambarus clarkii  Lake Caizi
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