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引用本文:谢薇薇,王志伟,孔繁翔,史小丽.太湖光合自养真核超微藻遗传多样性初探.湖泊科学,2012,24(1):123-128. DOI:10.18307/2012.0117
XIE Weiwei,WANG Zhiwei,KONG Fanxiang,SHI Xiaoli.Genetic diversity of photosynthetic picoeukaryotes in Lake Taihu. J. Lake Sci.2012,24(1):123-128. DOI:10.18307/2012.0117
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太湖光合自养真核超微藻遗传多样性初探
谢薇薇1,2, 王志伟1, 孔繁翔2, 史小丽2
1.南京农业大学生命科学学院, 南京 210095;2.中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008
摘要:
采用真核藻类叶绿体16S rRNA的引物PLA491F、OXY1313R,通过PCR、构建基因克隆库的方法初步调查了太湖梅梁湾和东太湖光合自养真核超微藻的遗传多样性.结果表明,太湖自养型真核超微藻主要为隐藻(Cryptophyta),其次为硅藻(Bacillariophyta)、金藻(Chrysophyta)和定鞭藻(Haptophyta).该结果有助于我们更全面地了解太湖浮游藻类的群落结构及生态功能.
关键词:  光合自养真核超微藻  叶绿体16S rRNA  遗传多样性  太湖
DOI:10.18307/2012.0117
分类号:
基金项目:国家自然科学基金项目(31070420);国家重点基础研究发展计划"973"项目(2008CB418005)联合资助
Genetic diversity of photosynthetic picoeukaryotes in Lake Taihu
XIE Weiwei1,2, WANG Zhiwei1, KONG Fanxiang2, SHI Xiaoli2
1.College of Life Science, Nanjing Agricultural University, Nanjing 210095, P. R. China;2.State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China
Abstract:
Genetic diversity of photosynthetic picoeukaryotes has been investigated at two stations located in Meiliang Bay and East Taihu in Lake Taihu, respectively. Genetic libraries of chloroplast 16S rRNA gene have been constructed by using primer set PLA491F/OXY1313R, biased towards chloroplast of marine algae. The results show that most of photosynthetic picoeukaryotic sequences belong to Cryptophyta, and the rest of them are affiliated with Bacillariophyta, Chrysophyta and Haptophyta. The study can help us take knowledge of the community structure and ecological function of phytoplankton in Lake Taihu.
Key words:  Photosynthetic picoeukaryotes  chloroplast 16S rRNA gene  genetic diversity  Lake Taihu
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