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引用本文:宋瑞峰,王国祥,徐瑶,邵继海,王中杰,刘洋,李仁辉.利用real-time RT-PCR研究大型蚤对铜绿微囊藻毒素合成基因转录水平影响.湖泊科学,2011,23(1):150-154. DOI:10.18307/2011.0122
SONG Ruifeng,WANG Guoxiang,XU Yao,SHAO Jihai,WANG Zhongjie,LIU Yang,LI Renhui.Transcriptional response of microcystin biosynthesis gene cluster of Microcystis aeruginosa PCC7806 under Daphnia stress using real-time RT-PCR technique. J. Lake Sci.2011,23(1):150-154. DOI:10.18307/2011.0122
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利用real-time RT-PCR研究大型蚤对铜绿微囊藻毒素合成基因转录水平影响
宋瑞峰1,2, 王国祥1, 徐瑶1,2, 邵继海2, 王中杰2, 刘洋2, 李仁辉2
1.南京师范大学地理科学学院, 江苏省环境演变与生态建设重点实验室, 南京 210046;2.中国科学院水生生物研究所, 武汉 430072
摘要:
近年来关于浮游动物与微囊藻相互作用的研究逐渐被关注, 其中有的研究认为浮游动物能够诱导产毒细胞毒素含量的变化.微囊藻毒素是由微囊藻毒素合成基因编码翻译的, 目前关于浮游动物对微囊藻毒素合成基因相对表达的影响并无报道, 本文首次通过实时定量逆转录PCR方法研究铜绿微囊藻PCC7806产毒相关基因mcyBmcyD在大型蚤胁迫下相对表达变化.结果显示微囊藻mcyBmcyD基因相对表达均有上调, 表明铜绿微囊藻PCC7806通过上调产毒基因的转录水平达到对大型蚤的诱导防御, 从而为浮游动物与微囊藻相互作用研究提供新的依据。
关键词:  real-time RT-PCR  微囊藻产毒基因  大型蚤  诱导防御
DOI:10.18307/2011.0122
分类号:
基金项目:国家水专项太湖课题(2009ZX07101-013-02)和中国科学院百人计划项目(082303-1-501)联合资助
Transcriptional response of microcystin biosynthesis gene cluster of Microcystis aeruginosa PCC7806 under Daphnia stress using real-time RT-PCR technique
SONG Ruifeng1,2, WANG Guoxiang1, XU Yao1,2, SHAO Jihai2, WANG Zhongjie2, LIU Yang2, LI Renhui2
1.Jiangsu Key Laboratory of Environmental Change and Ecological Construction, College of Geographical Science, Nanjing Normal University, Nanjing 210046, P.R.China;2.Institute of Hydrobioloby, Chinese Academy of Sciences, Wuhan 430072, P.R.China
Abstract:
Recently the interactions between zooplankton and Microcystis species have been concerned.Some studies have revealed that zooplankton can induce the change of toxin production in Microcystis.Microcystins(mcys) are cyclic heptapeptides, synthesized via a mixed polyketide synthase/nonribosomal petide synthetase system encoded by microcystin synthatase gene (mcy) operon, however no studies were conducted to elucidate the effect of zooplankton on the gene expressions of mcy genes.The present paper studied the effect of Daphnia upon the transcriptional regulation of genes responsible for microcystin synthesis in Murocystis aeruginosa PCC7806 using the real-time RT-PCR for the first time.Both mcyB and mcyD transcript levels increased under the grazing stress of Daphnia, and it is speculated that the increase of mcy gene clusters transcription was the induced defense of Microcystis aeruginosa PCC7806 against Daphnia stress.
Key words:  real-time RT-PCR  mcy gene clusters  Daphnia  induced defence
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