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引用本文:施丽梅,蔡元锋,杨华林,李朋富,孔繁翔,孔令东.太湖梅梁湾水华微囊藻基因型组成和产毒微囊藻丰度的变化.湖泊科学,2009,21(6):801-805. DOI:10.18307/2009.0608
SHI Limei,CAI Yuanfeng,YANG Hualin,LI Pengfu,KONG Fanxiang,KONG Lingdong.Dynamics of composition of different Microcystis spp. genotypes and abundance of toxic Microcystis in Meiliang Bay of Lake Taihu during bloom. J. Lake Sci.2009,21(6):801-805. DOI:10.18307/2009.0608
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太湖梅梁湾水华微囊藻基因型组成和产毒微囊藻丰度的变化
施丽梅1,2, 蔡元锋1, 杨华林1, 李朋富1,2, 孔繁翔2, 孔令东1
1.南京大学生命科学学院, 南京 210093;2.中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008
摘要:
采用基于ITS序列的PCR-DGGE方法分析了太湖梅梁湾水华暴发过程中(6-11月)微囊藻的不同基因型组成的变化,同时利用针对微囊藻毒素合成酶基因mcyA和微囊藻特异性的16SrDNA部分核苷酸序列的引物,应用实时荧光定量PCR方法(qRT-PCR)分析水华样品中产毒微囊藻与总微囊藻的量,通过两者的比值反映产毒微囊藻丰度.结果表明,在发生水华的不同时期,微囊藻的基因型组成发生了变化,其中以8月末、9月和10月的基因型最多,基因型M5和M10存在于整个水华发展过程中.共检测到12种主要的基因型,每个基因型在水华的不同时期所占的比例互不相同.实时荧光定量PCR结果显示从6月到10月,产毒微囊藻的丰度呈增大的趋势,从0.75%增加到32.16%,而11月产毒微囊藻的丰度显著下降.
关键词:  微囊藻  产毒微囊藻  ITS  实时荧光定量PCR  DGGE  水华  太湖
DOI:10.18307/2009.0608
分类号:
基金项目:国家重点基础研究发展计划(973)项目(2008CB418004);江苏省自然科学基金项目(BK2007150)联合资助
Dynamics of composition of different Microcystis spp. genotypes and abundance of toxic Microcystis in Meiliang Bay of Lake Taihu during bloom
SHI Limei1,2, CAI Yuanfeng1, YANG Hualin1, LI Pengfu1,2, KONG Fanxiang2, KONG Lingdong1
1.School of Life Sciences, Nanjing University, Nanjing 210093, P.R.China;2.State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P.R.China
Abstract:
Dynamics of composition of different Microcystis spp. genotypes from June to November, 2008 in Meiliang Bay of LakeTaihu in China were investigated based on denaturing gradient gel electrophoresis analysis of PCR-amplified ITS (internaltranscribed spacer sequence) fragments. Simultaneously, we investigated the relative abundance of microcystin-producingMicrocystis spp. (the ratio of the toxic Microcystis to total Microcystis) based on microcystin synthetase gene (mcyA) and partialMicrocystis-specific 16S rDNA sequence using real-time PCR. The results of this study indicated that there were shifts ofcomposition of different Microcystis genotypes during algae bloom in Lake Taihu. The most abundant genotypes were found in thelate of August, September and October. Genotypes M5 and M10 were found every month. A total of twelve main genotypes werefound, and the relative abundance of each genotype changed during the development of algae bloom. Quantitative real-time PCR(qRT-PCR) revealed that the abundance of toxic Microcystis increased from 0.75% to 32.16% from June to October and decreasedsignificantly in November.
Key words:  Microcystis  toxic Microcystis  ITS  qRT-PCR  DGGE  bloom  Lake Taihu
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