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引用本文:康丽娟,潘晓洁,常锋毅,李敦海,沈银武,刘永定.碱度增加对蛋白核小球藻光合活性与胞外多糖的影响.湖泊科学,2008,20(2):251-256. DOI:10.18307/2008.0218
KANG Lijuan,PAN Xiaojie,CHANG Fengyi,LI Dunhai,SHEN Yinwu,LIU Yongding.Effects of alkalinity variations on photosynthetic activity and exopotysaccharides of Chlorella pyrenoidosa. J. Lake Sci.2008,20(2):251-256. DOI:10.18307/2008.0218
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碱度增加对蛋白核小球藻光合活性与胞外多糖的影响
康丽娟1,2, 潘晓洁1,2, 常锋毅1,2, 李敦海2, 沈银武2, 刘永定2
1.中国科学院水生生物研究所, 武汉 430072;2.中国科学院研究生院, 北京 100049
摘要:
本文研究了不同重碳酸盐(HCO3-)碱度2.3mmol/L(ALK2.3)和12.4mmol/L(ALK12.4)条件对蛋白核小球藻光合活性、色素组成、丙二醛(MDA)含量与胞外多糖的影响.实验结果表明,碱度增加对蛋白核小球藻光合活性呈促进-抑制-促进效应,ALK2.3对光合活性影响的强度高于ALK12.4.碱度增加提高叶绿素b/叶绿素a(Chl.b/Chl.a)的值,降低类胡萝卜素/叶绿素(Caro/TChl)的值,并且ALK12.4条件下对藻细胞的作用程度强于ALK2.3.此外碱度增加刺激蛋白核小球藻胞外多糖分泌,ALK2.3在培养初期提高MDA含量,ALK12.4下细胞MDA含量显著降低.说明碱度增加会促进蛋白核小球藻光合活性,促进光合产物的积累与分泌.暗示胞外多糖的分泌可能是细胞适应高碱度的一种自我保护机制.
关键词:  碱度  光合活性  光合色素  胞外多糖  丙二醛
DOI:10.18307/2008.0218
分类号:
基金项目:中国科学院知识创新工程重大方向性项目(KZCX2-YW-426);国家重点基础研究发展“973”计划项目(2002CB412300,2002CB412306)联合资助
Effects of alkalinity variations on photosynthetic activity and exopotysaccharides of Chlorella pyrenoidosa
KANG Lijuan1,2, PAN Xiaojie1,2, CHANG Fengyi1,2, LI Dunhai1, SHEN Yinwu1, LIU Yongding1
1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China;2.Graduate School of Chinese Academy of Sciences, Beijing 100049, P. R. China
Abstract:
Effects of bicarbonate (HCO3-) alkalinity on the photosynthetic performance of Chlorella pyrenoidosa was investigated inpresent study. Photosynthetic activity, pigments, MDA and ultrastructure of Chlorella pyrenoidosa were measured for when they werecultured at two different alkalinities including 2.3mmol/L (ALK2.3) and 12.4mmol/L (ALK12.4), respectivily. At ALK2.3,photosynthetic activity was stimulated at the prophase of culture and then inhibited at the anaphase. At ALK12.4, it represented thesame phenomenon, but inhibition effects were weaker than those at ALK2.3. Ratios of Chl.b/Chl.a was increased, but Caro/T Chlratios was decreased at elevated alkalinities. The contents of MDA increased at ALK2.3 and decreased at ALK12.4. In addition,secretion of glycocalix-like layer was remarkably stimulated at elevated alkalinities. These results showed that elevated alkalinitiespromoted algae carbon assimilation and stimulated secretion of glycocalix-like layer. Carbohydrate could alleviate stresses at elevatedalkalinities. Secretion of glycocalix-like layer could decrease the synthesis of excess products of photosynthesis and relieve feedbackinhibition, which will increase the stress tolerance of C. pyenoidosa at elevated alkalinities.
Key words:  Alkalinity  photosynthesis  pigments  exopolysaccharides  malondialdehyde
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