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引用本文:杨钟,朱滨,常剑波.福尔马林固定白鲟(Psephurus gladius)标本的DNA提取及其相近种微卫星引物的适用性.湖泊科学,2008,20(2):242-250. DOI:10.18307/2008.0217
YANG Zhong,ZHU Bin,CHANG Jianbo.DNA extraction formalin-fixed tissue of Psephurus gladius and the applicability of microsatellite primers developed from its related species. J. Lake Sci.2008,20(2):242-250. DOI:10.18307/2008.0217
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福尔马林固定白鲟(Psephurus gladius)标本的DNA提取及其相近种微卫星引物的适用性
杨钟,朱滨,常剑波
1.水利部、中国科学院水工程生态研究所, 武汉 430079;2.中国科学院研究生院, 北京 100049
摘要:
白鲟是是世界上最大的淡水鱼之一,为我国独有,被誉为"长江中的活化石".目前白鲟群体已极度濒危,获取其新鲜材料非常困难,因此本文以保存于中科院水生所标本馆的福尔马林固定白鲟标本为研究材料,尝试从其肌肉、心脏、鳍条和肝脏组织提取白鲟基因组DNA,并检验相近种匙吻鲟和中华鲟微卫星引物对白鲟有无适用性.实验包括福尔马林固定白鲟标本从组织预处理到消化、抽提等步骤的整体优化过程;通过一系列对比试验分析在不同条件下提取到基因组DNA片段大小的差异,最终实现了福尔马林固定白鲟标本基因组DNA的稳定提取,并提出后续实验的延伸设想加以完善.
关键词:  白鲟  福尔马林  预处理  微卫星
DOI:10.18307/2008.0217
分类号:
基金项目:国家自然科学基金项目(30490234)资助
DNA extraction formalin-fixed tissue of Psephurus gladius and the applicability of microsatellite primers developed from its related species
YANG Zhong1,2,3, ZHU Bin1,2, CHANG Jianbo1,2
1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China;2.Institute of Hydroecology, Ministry of Water Resources & Chinese Academy of Sciences, Wuhan 430079, P. R. China;3.Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China
Abstract:
The Chinese paddlefish(Psephurus gladius(Martens))named as “the live fossil in the Yangtze River” is the indigenous species inChina, which is the biggest freshwater fish in the world. At present, the Chinese paddlefish has been in imminent danger, so it isextremely difficult to gain its fresh specimens. This research got the formalin-fixed tissues of Chinese paddlefish (11 individuals) asexperimental materials from the Institute of hydrobiology, Chinese Academy of Science. We have extracted the genomic DNA fromthe tissues of muscle, heart, fin and liver by optimizing a series of contrastive experiments, and consummated some researches onmolecular ecology by microsatellite molecular markers to realize its genetic characteristics.
Key words:  Chinese paddlefish  formalin  pretreatment  microsatellite
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