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引用本文:邬建勇,沈蕾,林国芳,沈建华,高光.太湖梅梁湾冬季表层水体致大肠杆菌阿拉伯糖抗性正向突变.湖泊科学,2002,14(4):337-342. DOI:10.18307/2002.0407
WU Jianyong,SHEN Lei,GAO Guang,LIN Gufang,SHEN Jianhua.The Mutagenicity Study of Surface Water from Meiliang Bay, Taihu Lake in Winter By E.coli UC1121 L-arabinose Resistance Forward Mutation Test. J. Lake Sci.2002,14(4):337-342. DOI:10.18307/2002.0407
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太湖梅梁湾冬季表层水体致大肠杆菌阿拉伯糖抗性正向突变
邬建勇1, 沈蕾1, 林国芳2, 沈建华1, 高光1
1.中国科学院上海生命科学研究院枯物生理生态研究所中德毒理学实验室, 上海200025;2.中国科学院南京地理与湖泊研究所太湖湖泊生态系统研究站, 南京210008
摘要:
本文采用阿拉伯糖抗性正向突变试验(ara试验)并辅以鼠伤寒沙门氏菌组氨酸营养缺陷回复突变试验(Ames试验)对太湖梅梁湾冬季表层水样品致突变性进行分析研究.ara试验结果表明9个点样品中有5个(主要为入湖河口及近岸样品)表现显著的直接致突变性,加入哺乳动物来源的代谢活化系统S9后,结果仍呈现同样的趋势.A-mes试验在增加两倍半上样剂量的条件下表现出与ara试验大体-致的检出率.在本研究中.ara试验对于成分复杂的环境样品表现更高的检出灵敏度.表明ara试验作为首选方法来检测环境中复杂化学物基因毒性方面有良好前景.
关键词:  太湖  致突变性  ara试验  Ames试验
DOI:10.18307/2002.0407
分类号:
基金项目:中国科学院创新项目(KZCX2-403)资助
The Mutagenicity Study of Surface Water from Meiliang Bay, Taihu Lake in Winter By E.coli UC1121 L-arabinose Resistance Forward Mutation Test
WU Jianyong1, SHEN Lei1, GAO Guang2, LIN Gufang1, SHEN Jianhua1
1.Sino-German Laboratory of Toxicology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200025, P. R. China;2.Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China
Abstract:
The L-arabinose resistance forward mutation test (ara test), together with Ames test, was performed for evaluation of mutagenicity of the surface water samples collected from Meiliang Bay, Taihu Lake in winter.The five of the nine surface water samples were detected as mutagen positive by ara test, most of them were collected from the estuary which flow into the lake or from the edge of shore area. The same tendency was observed with the involvement of mammalian S9 metabolic activation system.The roughly, coincident results were confirmed by Ames test when the sample doses applied were enhanced to two and a half times compared with that of ara test.The data suggest that detection sensitivity of ara lest is higher if intricate environmental samples are concerned.Thus, prospect for ara test to be the primary choice is prompted when the genotoxicity screening of environmental mixtures is required.
Key words:  Taihu Lake  mutagenicity  ara lest  Ames test
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