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引用本文:席贻龙,黄祥飞.培养液更换对萼花臂尾轮虫(Brachionus calyciflorus)休眠卵形成的影响.湖泊科学,2000,12(1):47-52. DOI:10.18307/2000.0107
XI Yilong,HUANG Xianfei.Effect of Culture Water Replacement on Resting Egg Formation of Freshwater Rotifer, Brachionus calyciflorus. J. Lake Sci.2000,12(1):47-52. DOI:10.18307/2000.0107
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培养液更换对萼花臂尾轮虫(Brachionus calyciflorus)休眠卵形成的影响
席贻龙, 黄祥飞
中国科学院水生生物研究所, 武汉430072
摘要:
在种群累积培养状态下研究了培养液更换对萼花臂尾轮虫(Brachionus calyciflorus)休眠卵形成的影响,结果表明,在培养液不予更换的情况下,萼花臂尾轮虫休眠卵的产量和形成效率分别为 724.3333±40.5257ind(6d.20mL)-1和 6.0361±0.3377ind(5×106 cells)-1(al-gae diet)与之相比,当培养液每 2天更换-次时,其休眠卵的产量和形成效率分别下降至637.3333±16.8028ind.(6d·20mL)-1和5.3111±0.1400ind(5×106cells)-1(algae diet).随着培养液更换频率增大至每天-次时,其休眠卵的产量和形成效率下降的幅度进-步增大培养液更换对轮虫种群中的混交雌体百分率和混交雌体受精率无显著的影响,最后探讨了培养液更换对萼花臂尾轮虫休眠卵形成影响的机理.
关键词:  萼花臂尾轮虫  培养液更换  休眠卵  产量  形成效率
DOI:10.18307/2000.0107
分类号:
基金项目:国家自然科学基金资助项目(39870158)
Effect of Culture Water Replacement on Resting Egg Formation of Freshwater Rotifer, Brachionus calyciflorus
XI Yilong, HUANG Xianfei
Institute of Hydrobiotogy, Chinese Academy of Sciences, Wuhan 430072, P. R. China
Abstract:
The effect of culture water replacement on resting egg formation of freshwater rotifer, Brachionus calyciflorus, was studied under conditions of population accumulative culture.The results showed that when the rotifer was cultured by batch culture method, the production and formation efficiency of resting egg were 724.3333±40.5257 ind.(6d·20mL)-11 and 6.0361±0.3377 ind.(5×106cells)-1 (algae diet), respectively.When the water replacement was made at every two days interval, the production and formation efficiency of resting egg decreased to 637.3333±16.8028 ind.(6d·20mL)-1 and 5.311±0.1400 ind.(5×106cells-1 (algae diet), respectively.The extent of the decline increased with the increment of water replacement frequency.There were no different effects of water replacement on the mixis and fertilization rate.The mechanism of the effects was also discussed.
Key words:  Brachionus calyciflorus  culture water replacement  resting egg  production  formation efficiency
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