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引用本文:陶明煊,吴国荣,陆长梅,程光宇,魏锦城.极大螺旋藻铁型超氧物歧化酶的纯化及性质.湖泊科学,1999,11(1):63-69. DOI:10.18307/1999.0110
TAO Ming-xuan,WU Guo-rong,LU Chang-mei,CHENG Guang-yu,WEI Jin-cheng.Purification and Properties of Ironcontaining Superoxide Dismutase from Spirulina maxima. J. Lake Sci.1999,11(1):63-69. DOI:10.18307/1999.0110
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极大螺旋藻铁型超氧物歧化酶的纯化及性质
陶明煊, 吴国荣, 陆长梅, 程光宇, 魏锦城
南京师范大学生物系, 南京 210097
摘要:
极大螺旋藻 Spirulina maxima藻体SOD聚丙烯酰胺梯度凝胶电泳呈现4条同工 酶带, H2O2明显抑制该酶活性, KCN对酶活性无影响, 确认为 Fe-SOD经硫酸铵分部盐析、离子交 换柱层析及凝胶过滤, 纯化到电泳单斑点均-程度.纯化的Fe-SOD分子量为39.3KD, 亚基分子 量为20KD金属元素分析表明, 每个亚基含0 55个Fe原子, 该酶在紫外区最大吸收峰值为 275.8nm、该酶氨基酸组成与蓝、绿藻和高等植物的Fe-SOD相似, 但它含有较高的丙氨酸, 酸性氨 基酸和碱性氨基酸比值与低等植物及原核生物相近而明显高于高等植物.
关键词:  极大螺旋藻  铁型超氧物歧化酶  纯化
DOI:10.18307/1999.0110
分类号:
基金项目:江苏省自然科学基金, 1997SWX0010SK1
Purification and Properties of Ironcontaining Superoxide Dismutase from Spirulina maxima
TAO Ming-xuan, WU Guo-rong, LU Chang-mei, CHENG Guang-yu, WEI Jin-cheng
Biological Department, Nanjing Normal University, Nanjing 210097
Abstract:
The isozyme of SOD in Spirulina maxima with four bands by gradient electrophoresis was identified as Fe-SOD, which was purified by ammonium sulfate fractionation followed by DEAE-Celluose and Sephadex G-100 column chromatography.The molecular weight of the enzyme is 39.3KD, and that of its subunit molecular mass is 20KD.Metal analysis showed that the Fe-SOD contains about 0.55 atom of iron per subunit.The enzyme exhibits one absorption maximum at 275.8nm.The enzyme activity could be inhibited by H2O2 but unaffected by KCN.The Fe-enz-yme contains more alanine residues.The ratio of acid amino acid and alkaline amino acid is similar to lower plants and procaryotes, obviously higher than those of higher plants.
Key words:  Spirulina maxima  iron-containing superoxide dismutase  purification
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