| 引用本文: | 张靖雯,毛致伟,杨江华,金小伟,张效伟.环境DNA技术在两栖动物监测中的研究与应用进展.湖泊科学,2025,37(4):1162-1174. DOI:10.18307/2025.0409 |
| Zhang Jingwen,Mao Zhiwei,Yang Jianghua,Jin Xiaowei,Zhang Xiaowei.Research and application progress of environmental DNA technology in amphibian monitoring. J. Lake Sci.2025,37(4):1162-1174. DOI:10.18307/2025.0409 |
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| 摘要: |
| 两栖动物是全球受威胁最严重的脊椎动物类群，其种群衰退主要源于栖息地丧失、气候变化、环境污染、入侵物种及病菌感染等多重压力。为提升两栖动物多样性保护成效，建立高效精准的监测技术以科学评估其生存现状和动态变化至关重要。传统形态学和声学监测方法虽然应用广泛，但存在监测成本高、鉴定难度大、难以发现隐蔽稀有物种等局限。环境DNA（eDNA）技术以其高效、快速、无创的优势，为两栖动物多样性监测提供了新的解决方案。本文简要介绍了eDNA生物监测的两种主要方法，综述了eDNA技术在两栖动物“靶向”（入侵物种、珍稀/濒危物种）监测、生物多样性调查、物种丰度估测以及致病菌监测方面的应用进展，重点探讨了样品采集与eDNA捕获、引物选择与PCR扩增、生物信息学分析、假阴性与假阳性的控制等关键技术环节对监测准确性的影响，并提出了优化建议。此外，本文对未来的研究方向与应用前景进行了展望，以期为两栖动物多样性监测、保护与管理提供参考。 |
| 关键词: 两栖动物 环境DNA 生物监测 宏条形码 生物多样性 |
| DOI：10.18307/2025.0409 |
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| 文献标识码:A |
| 基金项目: |
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| Research and application progress of environmental DNA technology in amphibian monitoring |
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Zhang Jingwen1，Mao Zhiwei1，Yang Jianghua2，Jin Xiaowei3，Zhang Xiaowei4
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1:Tianjin Hexi Ecological Environment Monitoring Center, Tianjin 300201 , P.R.China ,2:School of Marine Science and Engineering, Nanjing Normal University, Nanjing 210046 , P.R.China,3:China National Environmental Monitoring Center, Beijing 100012 , P.R.China ,4:School of Ecology and Environment, Yunnan University, Kunming 650091 , P.R.China
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| Abstract: |
| Amphibians represent the most threatened vertebrate group on the planet, with their population decline being primarily attributable to a number of pressures including, but not limited to, habitat loss, climate change, environmental pollution, the introduction of invasive species and disease infection. In order to enhance the efficacy of amphibian diversity conservation, it is imperative to establish efficient and accurate monitoring techniques to scientifically evaluate the status and dynamics of amphibian populations. Despite the prevalence of conventional morphological and acoustic monitoring techniques, they are encumbered by inherent limitations, including the high financial cost of monitoring, the complexity of species identification, and the challenge of detecting rare species. The utilisation of environmental DNA (eDNA) technology has emerged as a novel approach for the assessment of amphibian diversity, exhibiting notable advantages such as high efficiency, expeditiousness, and non-invasiveness. This paper provides a concise overview of the two primary methods of eDNA biomonitoring, offering a synopsis of the advancements in eDNA technology for the purpose of amphibian “targeted” (invasive species, rare/endangered species) monitoring, biodiversity surveys, species abundance estimation, and pathogenic bacteria monitoring. The focus of this research is the impact of key technical aspects on the accuracy of monitoring, including but not limited to: sample collection and eDNA capture, primer selection and PCR amplification, bioinformatics analysis, and the control of false negatives and false positives. Suggestions for the optimisation of these processes are also put forward. Furthermore, the subsequent research directions and potential applications are discussed in this paper, with the aim of providing a framework for the future monitoring, conservation and management of amphibian diversity. |
| Key words: Amphibian environmental DNA biomonitoring metabarcoding biodiversity |
