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引用本文:赵子明,叶建生,刘美剑,沈冬冬,黄世熠.五个湖泊中华刺鳅(Mastacembelus sinensis)种群线粒体细胞色素b遗传多样性分析.湖泊科学,2016,28(5):1031-1038. DOI:10.18307/2016.0513
ZHAO Ziming,YE Jiansheng,LIU Meijian,SHEN Dongdong,HUANG Shiyi.Genetic diversity of spiny eel (Mastacembelus sinensis) from five lakes based on Mitochondrial DNA cyt b gene. J. Lake Sci.2016,28(5):1031-1038. DOI:10.18307/2016.0513
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五个湖泊中华刺鳅(Mastacembelus sinensis)种群线粒体细胞色素b遗传多样性分析
赵子明, 叶建生, 刘美剑, 沈冬冬, 黄世熠
江苏农牧科技职业学院, 泰州 225300
摘要:
采用特异性引物对中华刺鳅基因组DNA进行扩增和测序,获得了江苏省5个湖泊的中华刺鳅种群线粒体cyt b基因767 bp的序列.分析表明,cyt b基因中A+T含量略高于G+C含量,144个中华刺鳅共获得cyt b基因单倍型28个,变异位点38个,其中单倍型Hap1分布最为广泛,可能为祖先型单倍型.平均单倍型多样性和平均核苷酸多样性指数分别为0.58751和0.00214,遗传多样性较低.群体间遗传分化系数为0.0356,绝大多数的遗传变异都来自群体内部,而群体间的遗传分化极小.构建的系统进化树未出现明显的以单个湖泊群体的聚簇,5个湖泊的种群间基因交流极其强烈.研究结果表明,当前5个湖泊的中华刺鳅遗传多样性水平比较低,亟待加强保护.
关键词:  中华刺鳅  线粒体DNA  cyt b  遗传多样性  淡水湖
DOI:10.18307/2016.0513
分类号:
基金项目:江苏农牧科技职业学院课题(NSFRC1304)资助.
Genetic diversity of spiny eel (Mastacembelus sinensis) from five lakes based on Mitochondrial DNA cyt b gene
ZHAO Ziming, YE Jiansheng, LIU Meijian, SHEN Dongdong, HUANG Shiyi
Jiangsu Agri-animal Husbandry Vocational College, Taizhou 225300, P. R. China
Abstract:
The mitochondrial DNA cyt b gene (767 bp) of spiny eel from five lakes in Jiangsu Province was amplified and sequenced. The average contents of A+T were a little higher than those of G+C when 38 variable sites were detected, and 28 haplotypes were recovered. The average haplotype diversity (0.58751) and nucleotide diversity (0.00214) demonstrated very low genetic diversity of the spiny eel populations in the five lakes. The fixation indice of analysis of molecular variance was 0.0356, which showed no significant genetic difference among populations. The UPGMA phylogenetic tree of cyt b gene built with MEGA6.0 showed that five populations were not clustered into clades based on genetic distance. There was an extensive general flow among spiny eel populations from the five lakes in Jiangsu Province. The result showed that spiny eel from the five lakes had low genetic diversity and genetically homogeneous population structure, indicating their weak viability to maintain population. The spiny eel need to be well protected.
Key words:  Mastacembelus sinensis  Mitochondrial DNA  cyt b  genetic diversity  freshwater lakes
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