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引用本文:于婷,戴景峻,雷腊梅,彭亮.温度、光照强度及硝酸盐对拟柱孢藻(Cylindrospermopsis raciborskii N8)生长的影响.湖泊科学,2014,26(3):441-446. DOI:10.18307/2014.0315
YU Ting,DAI Jingjun,LEI Lamei,PENG Liang.Effects of temperature, irradiance and nitrate on the growth of Cylindrospermopsis raciborskii N8. J. Lake Sci.2014,26(3):441-446. DOI:10.18307/2014.0315
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温度、光照强度及硝酸盐对拟柱孢藻(Cylindrospermopsis raciborskii N8)生长的影响
于婷, 戴景峻, 雷腊梅, 彭亮
暨南大学水体富营养化与赤潮防治广东普通高校重点实验室, 广州 510632
摘要:
以从南亚热带水库中分离的拟柱孢藻(Cylindrospermopsis raciborskii N8)为材料,研究了不同温度(12、16、20、24、28、32℃)、光照强度(6.6、12.4、21.5、30.7、62.9、106.4μmol/(m2·s))和硝态氮浓度(0.5、1、2、4、8、16、32、64、128 mg/L)下拟柱孢藻的生长特性.结果表明:在实验设置的温度范围(16~32℃)内拟柱孢藻能够正常生长;最适温度范围为24~28℃,在28℃条件下,具有最大比生长速率,为0.189 d-1;当温度为12℃时,拟柱孢藻叶绿素a浓度一直降低,显著低于其他温度组(16~32℃).在6.6~106.4μmol/(m2·s)光照强度范围内,拟柱孢藻均呈指数增长趋势,最适光照强度为30.7μmol/(m2·s),其比生长速率达到最大值,为0.156 d-1;高光照强度(62.9~106.4μmol/(m2·s))下拟柱孢藻的比生长速率显著大于低光照强度(6.6~12.4μmol/(m2·s))处理组.拟柱孢藻开始指数增长的最低硝态氮浓度为4 mg/L;硝态氮浓度为8 mg/L时,拟柱孢藻达到最大比生长速率(0.155 d-1);当硝态氮浓度高于16 mg/L时比生长速率增加不显著.高硝态氮浓度组(16~128 mg/L)拟柱孢藻的叶绿素a浓度和比生长速率显著高于低硝态氮浓度组(0.5~2 mg/L).研究结果说明拟柱孢藻对温度、光照和氮源均有较宽的生态位,有利于在较大空间尺度上进行扩散.
关键词:  拟柱孢藻  生长  比生长速率  温度  光照强度  硝酸盐
DOI:10.18307/2014.0315
分类号:
基金项目:国家自然科学基金项目(31070416);暨南大学科研培育与创新基金项目(11612322);国家级大学生创新创业训练计划项目(1210559020)联合资助
Effects of temperature, irradiance and nitrate on the growth of Cylindrospermopsis raciborskii N8
YU Ting, DAI Jingjun, LEI Lamei, PENG Liang
Department of Ecology and Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou 510632, P. R. China
Abstract:
Cylindrospermopsis raciborskii N8 strain was isolated from a sub-tropical reservoir in southern China and cultured with BG-11 culture medium. The effects of temperature, irradiance and nitrate on the growth of this strain were evaluated by a designed experiment with gradients of temperature (12, 16, 20, 24, 28, 32℃), irradiance(6.6, 12.4, 21.5, 30.7, 62.9, 106.4 μmol/(m2·s)) and nitrate (0.5, 1, 2, 4, 8, 16, 32, 64, 128 mg/L). C. raciborskii N8 had normal growth at temperature between 16℃ and 32℃, and the maximum specific growth rate (0.189 d-1) were observed at temperature between 24℃ and 28℃; C. raciborskii N8 could not survive at 12℃, and chlorophyll-a concentration was significantly lower than other temperature treatments (16-32℃). C. raciborskii N8 had a wide adaptation of irradiance ranging from 6.6 μmol/(m2·s) to 106.4 μmol/(m2·s). An exponential growth was found from 6.6 to 106.4 μmol/(m2·s). The optimal irradiance was 30.7 μmol/(m2·s) and the maximum specific growth rate was 0.156 d-1. C. raciborskii N8 grew faster significantly in high irradiance conditions (62.9-106.4 μmol/(m2·s)) than that in low irradiance conditions (6.6-12.4 μmol/(m2·s)). The species began an exponential growth under nitrate of 4 mg/L, reached the highest specific growth rate (0.155 d-1) at the nitrate concentration of 8 mg/L. The specifical growth rates became stable when the nitrate concentration exceeded 16 mg/L. C. raciborskii N8 grew faster significantly in the high nitrate conditions (16-128 mg/L) than that in the low nitrate conditions (0.5-2 mg/L). The present experiments demonstrated that C. raciborskii N8 can adapt for wide temperatures, irradiance and nitrates, and has a potential to colonize and distribute in divers water body over a large scale.
Key words:  Cylindrospermopsis raciborskii N8  growth  specific growth rate  temperature  irradiance  nitrate
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