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引用本文:赵吉睿,巩瑞红,李畅游,王瑞刚,陈晨,陈勿力吉玛,牛晓峰,冯福应.三种碳源对乌梁素海好氧不产氧光合细菌群落结构的影响.湖泊科学,2014,26(1):113-120. DOI:10.18307/2014.0114
ZHAO Jirui,GONG Ruihong,LI Changyou,WANG Ruigang,CHEN Chen,CHEN Wulijima,NIU Xiaofeng,FENG Fuying.Influence of three kinds of carbon source on community structure of aerobic anoxygenic phototrophic bacteria in Lake Ulansuhai. J. Lake Sci.2014,26(1):113-120. DOI:10.18307/2014.0114
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三种碳源对乌梁素海好氧不产氧光合细菌群落结构的影响
赵吉睿1, 巩瑞红1, 李畅游2, 王瑞刚1, 陈晨1, 陈勿力吉玛1, 牛晓峰3, 冯福应1
1.内蒙古农业大学生命科学学院应用与环境微生物研究所, 呼和浩特 010018;2.内蒙古农业大学水利与土木建筑工程学院, 呼和浩特 010018;3.内蒙古自治区赤峰市喀喇沁旗锦山镇人民政府, 赤峰 024400
摘要:
好氧不产氧光合细菌(AAPB)营光合异养,在水体碳循环中起着重要作用.但是,碳源对AAPB群落结构的影响尚不明确.本文基于16S rDNA和光合反应中心合成中的关键基因——pufM基因,运用变性梯度凝胶电泳(DGGE)技术,比较分析了在微生物分离培养中常用的3种碳源(葡萄糖、丙酮酸钠、酵母提取物)对乌梁素海水体总细菌及AAPB群落结构的影响.结果表明,不同碳源诱导7~21 d后,水体总细菌群落丰富度及多样性指数均有不同程度的提升,AAPB群落则呈现先升后降的趋势.3种碳源中,丙酮酸钠添加后对总细菌及AAPB菌群的丰富度和多样性的提升效果最好.测序及系统发育结果表明,乌梁素海水体经诱导后新检测到了一些原环境中未发现的菌属,一些具有特殊碳代谢能力的菌属丰度得到了提升.本研究为充分认识和理解AAPB的生态学作用和意义,及其富集、分离和培养提供了基础依据.
关键词:  好氧不产氧光合细菌  碳源  群落结构  变性梯度凝胶电泳  乌梁素海
DOI:10.18307/2014.0114
分类号:
基金项目:国家自然科学基金项目(30760004);内蒙古农业大学科技创新团队基金项目(NDPYTD2010-3)联合资助
Influence of three kinds of carbon source on community structure of aerobic anoxygenic phototrophic bacteria in Lake Ulansuhai
ZHAO Jirui1, GONG Ruihong1, LI Changyou2, WANG Ruigang1, CHEN Chen1, CHEN Wulijima1, NIU Xiaofeng3, FENG Fuying1
1.Institute for Applied and Environmental Microbiology of Life Sciences College, Inner Mongolia Agricultural University, Huhhot 010018, P. R. China;2.Water Conservancy and Civil Engineering College, Inner Mongolia Agricultural University, Huhhot 010018, P. R. China;3.People's Government of Jinshan Town in Harcin County, Chifeng 024400, P. R. China
Abstract:
Aerobic anoxygenic phototrophic bacteria (AAPB) are photoheterotrophic microbes and play a significant role in carbon cycle in aquatic ecosystems. However, the effect of carbon source on the community structure of AAPB remains unknown presently. This study determined the influence of three carbon sources, glucose, sodium pyruvate and yeast extract common used in microbe isolation and culture on total bacteria and AAPB community structures of Lake Ulansuhai, using denaturing gradient gel electrophoresis based on the genes of 16S rDNA and pufM of photosynthetic reaction center respectively. The results showed that diversity and richness of total bacteria community had been enhanced while those of AAPB community been improved previous to 14 days then decreased, after inducing by different carbon sources for 7-21days. Among the three carbon sources, sodium pyruvate best increased the richness and diversity of both total bacteria and AAPB. Sequencing and phylogenetic analysis revealed that some taxonomic strains were discovered which failed to be determined before induction by carbon source and moreover the abundance of some strains harboring specific carbon metabolism were greatly elevated. These results may promote the appreciation for the function and significance of AAPB and provide the basis for their enrichment, isolation and culture.
Key words:  Aerobic anoxygenic phototrophic bacteria  carbon source  community structure  denaturing gradient gel electrophoresis  Lake Ulansuhai
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