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引用本文:姜岩,施国新,陈霖,汪鹏合,乔绪强,田秀丽.铜胁迫对菹草无菌苗多胺及活性氧代谢的影响.湖泊科学,2013,25(4):551-557. DOI:10.18307/2013.0413
JIANG Yan,SHI Guoxin,CHEN Lin,WANG Penghe,QIAO Xuqiang,TIAN Xiuli.Effect of copper stress on polyamine and reactive oxygen species metabolism in aseptic seedlings of Potamogeton crispus L.. J. Lake Sci.2013,25(4):551-557. DOI:10.18307/2013.0413
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铜胁迫对菹草无菌苗多胺及活性氧代谢的影响
姜岩, 施国新, 陈霖, 汪鹏合, 乔绪强, 田秀丽
南京师范大学生命科学学院, 江苏省生物多样性与生物技术重点实验室, 南京 210046
摘要:
本文研究了不同浓度Cu2+(0、1、2、4、8、12μmol/L)对菹草无菌苗多胺(PAs)及活性氧代谢的影响.结果表明,随着Cu2+处理浓度的升高:(1)菹草无菌苗植株的超氧化物歧化酶(SOD)活性逐渐上升,过氧化物酶(POD)和过氧化氢酶(CAT)活性则表现为先升后降;(2)超氧阴离子(O2.-)产生速率逐渐增大,过氧化氢(H2O2)和丙二醛(MDA)有所积累,可溶性蛋白含量降低;(3)游离态多胺含量与多胺总含量的变化一致,均表现为腐胺(Put)含量先升后降,亚精胺(Spd)含量逐渐降低,精胺(Spm)含量逐渐上升;游离态多胺(Spd+Spm)/Put的比值在高浓度Cu2+胁迫时迅速上升;结合态Put和Spm的含量变化与游离态的变化一致,而结合态Spd的含量与对照相比均略有上升;束缚态Put和Spd含量先升后降,而Spm含量则逐渐降低;(4)精氨酸脱羧酶(ADC)、多胺氧化酶(PAO)和二胺氧化酶(DAO)活性均表现为先升后降,而鸟氨酸脱羧酶(ODC)活性变化不明显.可见,Cu2+对菹草无菌苗造成了明显的氧化胁迫,抗氧化酶活性的升高,Spm含量的升高,Put向Spd和Spm的转化以及不同状态多胺之间的转化,在菹草无菌苗抵抗Cu2+胁迫过程中发挥着重要的作用.
关键词:  菹草  无菌苗  Cu2+胁迫  活性氧  多胺代谢
DOI:10.18307/2013.0413
分类号:
基金项目:江苏高校优势学科建设工程项目(164320H106);国家自然科学基金项目(30870139)联合资助
Effect of copper stress on polyamine and reactive oxygen species metabolism in aseptic seedlings of Potamogeton crispus L.
JIANG Yan, SHI Guoxin, CHEN Lin, WANG Penghe, QIAO Xuqiang, TIAN Xiuli
Jiangsu Key Lab of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China
Abstract:
Taking aseptic seedlings of P.crispus as the experimental material,the effect of different concentrations of Cu2+ (0,1, 2,4,8,12 μmol/L) on the metabolism of polyamine(PAs) and reactive oxygen were investigated.With the increasing of Cu2+ concentration,the results indicated that: (1) Superoxide dismutase(SOD) activity increased markedly,while the activities of peroxidase(POD) and catalase(CAT) increased progressively followed by a decline.(2) The generation rate of O2-· and the content of H2O2 and MDA increased,while soluble protein content decreased.(3) Free putrescine(Put) content first enhanced and then reduced notably.However,the content of free spermidine(Spd) decreased gradually and spermine(Spm) increased significantly.Therefore,the ratio of free (Spd+ Spm)/Put promptly increased under high concentrations of Cu2+ stress.The changes of total PAs content were similar to those on free PAs.Perchloric acid soluble conjugated(PS-conjugated) Put and Spm changed in the same pattern as free PAs.While PS-conjugated Spd content increased slightly.In addition,the content of perchloric acid insoluble bound(PIS-bound) Put and Spd first increased and then decreased,but they were still higher than those in the control.Moreover, PIS-bound Spm content declined.(4) With the increase of Cu2+ concentration,the activity of arginine decarboxylase (ADC) sharply decreased after a transient increase,while the activity of ornithine decarboxylase(ODC) did not change significantly.Polyamine oxidase(PAO) and diamine oxidase(DAO) activities increased gradually,followed by a decrease.Our results suggested that Cu2+ stress induced obvious oxidative damage on aseptic seedlings of P.crispus.The up-regulation of antioxidant enzymes activities and Spm content,the changes of Put into Spd and Spm as well as the conversion between different forms of polyamines played a key role in the adaptation mechanism of aseptic seedlings of P.crispus under Cu2+ stress.
Key words:  Potamogeton crispus L.  aseptic seedling  Cu2+ stress  reactive oxygen  polyamine metabolism
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