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引用本文:钟立强,刘朋朋,潘建林,王明华,陈友明,秦钦,边文冀,陈校辉.长江中下游5个湖泊黄颡鱼(Pelteobagrus fulvidraco)种群线粒体细胞色素b基因的遗传变异分析.湖泊科学,2013,25(2):302-308. DOI:10.18307/2013.0219
ZHONG Liqiang,LIU Pengpeng,PAN Jianlin,WANG Minghua,CHEN Youming,QIN Qin,BIAN Wenji,CHEN Jiaohui.Genetic variation analysis of yellow catfish (Pelteobagrus fulvidraco) from five lakes in the middle and lower reaches of the Yangtze River based on Mitochondrial DNA cyt b. J. Lake Sci.2013,25(2):302-308. DOI:10.18307/2013.0219
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长江中下游5个湖泊黄颡鱼(Pelteobagrus fulvidraco)种群线粒体细胞色素b基因的遗传变异分析
钟立强1, 刘朋朋2, 潘建林1,2, 王明华1, 陈友明1, 秦钦1, 边文冀1, 陈校辉1
1.江苏省淡水水产研究所, 南京 210017;2.南京师范大学生命科学学院, 南京 210097
摘要:
对长江中下游5个湖泊的黄颡鱼种群的线粒体DNA细胞色素b基因进行了PCR扩增、测序,获得了955 bp的序列.分析显示,cyt b序列中A+T含量略高于G+C含量,共检测到54个变异位点,60个样本得到37个单倍型,平均单倍型多样性为0.945±0.018,核苷酸多样性为0.00419±0.00043,遗传多样性表现中等.太湖种群与滆湖种群间的遗传距离最远为0.00651,鄱阳湖种群和巢湖种群之间遗传距离最近为0.00375.分子方差分析(AMOVA)表明,群体间遗传分化系数Fst为0.0684,几乎所有变异都来自群体内,群体间遗传分化极小.cyt b序列构建UPGMA系统进化树显示,5个种群没有分化成不同的分枝谱系,种群间存在广泛的基因交流.
关键词:  黄颡鱼  线粒体DNA  细胞色素b基因  遗传变异  长江中下游
DOI:10.18307/2013.0219
分类号:
基金项目:江苏省科技支撑计划项目(BE2010376);江苏省农业科技自主创新基金项目[CX(11)1036]联合资助
Genetic variation analysis of yellow catfish (Pelteobagrus fulvidraco) from five lakes in the middle and lower reaches of the Yangtze River based on Mitochondrial DNA cyt b
ZHONG Liqiang1, LIU Pengpeng2, PAN Jianlin1,2, WANG Minghua1, CHEN Youming1, QIN Qin1, BIAN Wenji1, CHEN Jiaohui1
1.Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, P.R.China;2.School of Life Science, Nanjing Normal University, Nanjing 210097, P.R.China
Abstract:
The mtDNA cyt b gene of yellow catfish from five lakes in the middle and lower reaches of the Yangtze River was amplified and sequenced.Result showed that the average contents of A+T were a little higher than that of G+C in the aligned sequences of 955 bp.54 variable sites were detected and 37 haplotypes were recovered.The haplotype diversity and nucleotide diversity were 0.945±0.018 and 0.00419±0.00043,respectively.The genetic distance between Lake Taihu population and Lake Gehu population displayed the highest,up to 0.00651,while that between Lake Poyang and Lake Chaohu was the lowest to 0.00375.The fixation indices (Fst) of analysis of molecular variance (AMOVA) among populations was 0.0684,illustrating no significant population structure.The phylogenetic tree of cyt b built with MEGA 4.1 showed that five populations were not clustered into clades based on genetic distance.There was extensive general flow between yellow catfish populations from five lakes in the middle and lower reaches of the Yangtze River.
Key words:  Pelteobagrus fulvidraco  Mitochondrial DNA  cyt b  genetic diversity  middle and lower reaches of the Yangtze River
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