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引用本文:张静,韦玉春,王国祥,杨飞,程春梅,夏晓瑞.太湖蓝藻水样中藻蓝蛋白提取方法比较.湖泊科学,2013,25(2):283-288. DOI:10.18307/2013.0216
ZHANG Jing,WEI Yuchun,WANG Guoxiang,YANG Fei,CHENG Chunmei,XIA Xiaorui.Comparison of extraction methods for phycocyanin from cyanobacterica blooms water samples in Lake Taihu. J. Lake Sci.2013,25(2):283-288. DOI:10.18307/2013.0216
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太湖蓝藻水样中藻蓝蛋白提取方法比较
张静1, 韦玉春1, 王国祥2, 杨飞2, 程春梅1, 夏晓瑞1
1.南京师范大学虚拟地理环境教育部重点实验室, 南京 210046;2.江苏省环境演变与生态建设重点实验室, 南京 210046
摘要:
以2011年8月20日采集的太湖梅梁湾的夏季蓝藻水华为研究对象,通过12个样点平行样的藻蓝蛋白实验提取,基于光谱吸收特征和浓度值,对反复冻融法、超声波法、溶胀法、丙酮法的提取效果进行比较评价.结果表明:4种方法获取的藻蓝蛋白提取液在620 nm附近出现吸收峰,其中,反复冻融法的峰高最强,超声波法最弱,说明反复冻融法的提取效果优于其他方法;反复冻融法、超声波法、溶胀法获取的部分蓝蛋白提取液在670 nm附近具有次吸收峰,与藻蓝蛋白标样的吸收曲线存在差异;反复冻融法、超声波法提取的藻蓝蛋白浓度值变异系数小于0.6,表明这两种方法较其他方法稳定;反复冻融法提取的藻蓝蛋白浓度值高于其他3种方法,可推荐作为太湖蓝藻水样中藻蓝蛋白的提取方法.
关键词:  藻蓝蛋白  提取方法  太湖  梅梁湾  蓝藻水华  分光光度法
DOI:10.18307/2013.0216
分类号:
基金项目:国家自然科学基金项目(40771152);江苏省普通高校自然科学研究计划项目(09KJA420001);江苏高校优势学科建设工程项目(1411109012)联合资助
Comparison of extraction methods for phycocyanin from cyanobacterica blooms water samples in Lake Taihu
ZHANG Jing1, WEI Yuchun1, WANG Guoxiang2, YANG Fei2, CHENG Chunmei1, XIA Xiaorui1
1.Key Lab of Virtual Geographic Environment, Ministry of Education, Nanjing Normal University, Nanjing 210046, P.R.China;2.Jiangsu Key Laboratory of Environmental Change and Ecological Construction, Nanjing 210046, P.R.China
Abstract:
Taking cyanobacterica blooms water samples collected from 12 different sites in Meiliang Bay of Lake Taihu in August 20,2011 as a research object,the phycocyanin extraction method including freeze thawing cycle,ultrasonic wave extracting,cellmelting and acetone were compared based on absorption spectrum and phycocyanin concentration.Results showed that the absorption spectrum of phycocyanin extracted by these methods have significant peak at 620 nm.The peak height is quite different among these methods: the highest peak is observed in freeze thawing cycle while the lowest in ultrasonic wave extracting,indicating that the four methods can extract phycocyanin from water samples,and freeze thawing cycle method is superior to others;Some phycocyanin specturm obtained by freeze thawing cycle,ultrasonic wave extracting and cell-melting appeared the second peak near 670 nm,and differentiated with the specturm of phycocyanin standard sample;The variation coefficient of phycocyanin concentration in the freeze thawing cycle method and ultrasonic wave method are less than 0.6,showing that their result is more precise and stable;The phycocyanin concentration showed that extraction efficiency of freeze thawing cycle method is better than the others,and so is recommended as a common method for phycocyanin extraction from cyanobacterica blooms water samples in Lake Taihu.
Key words:  Phycocyanin  extraction methods  Lake Taihu  Meiliang Bay  cyanobacterica blooms  spectrophotometry
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