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引用本文:邓洁,李建宏,管章玲,胡碧洋,赵蕾,李朋富.一株产碳酸酐酶附生菌对铜绿微囊藻(Microcystis aeruginosa)生长的影响.湖泊科学,2012,24(3):429-435. DOI:10.18307/2012.0315
DENG Jie,LI Jianhong,GUAN Zhangling,HU Biyang,ZHAO Lei,LI Pengfu.Effect of attached bacteria of carbonic anhydrase on the growth of Microcystis aeruginosa. J. Lake Sci.2012,24(3):429-435. DOI:10.18307/2012.0315
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一株产碳酸酐酶附生菌对铜绿微囊藻(Microcystis aeruginosa)生长的影响
邓洁1, 李建宏1, 管章玲1, 胡碧洋1, 赵蕾1, 李朋富2
1.南京师范大学生命科学学院, 南京 210046;2.南京大学生命科学学院, 南京 210093
摘要:
为研究生长环境中微生物对铜绿微囊藻碳代谢的影响,本文分析太湖典型微囊藻水华样品附生菌中产碳酸酐酶(CA)细菌的比例,结果显示CA菌占11.6%;从微囊藻群体中分离获得了一株高胞外CA附生菌P201,通过ITS基因鉴定,该菌为一株荧光假单胞菌(Pseudomonas fluorescence).并研究了该菌在不同浓度HCO3-条件下对铜绿微囊藻生长的影响,结果表明无论是高HCO3-浓度还是低HCO3-浓度环境中,加入该菌对铜绿微囊藻的生长均有促进作用,说明产CA酶附生菌对铜绿微囊藻的生长有一定的促进作用.
关键词:  铜绿微囊藻  荧光假单胞菌  附生菌  碳酸酐酶
DOI:10.18307/2012.0315
分类号:
基金项目:国家重点基础研究发展计划项目(2008CB418004);国家基础科学人才培养基金项目(J1103507);江苏高校优势学科建设工程项目联合资助
Effect of attached bacteria of carbonic anhydrase on the growth of Microcystis aeruginosa
DENG Jie1, LI Jianhong1, GUAN Zhangling1, HU Biyang1, ZHAO Lei1, LI Pengfu2
1.Life Sciences College of Nanjing Normal University, Nanjing 210046, P. R. China;2.School of Life Science, Nanjing University, Nanjing 210093, P. R. China
Abstract:
To study the effect of attached bacteria on the carbon metabolism of M. aeruginosa, the percentage of carbonic anhydrase(CA) produced bacteria in total attached bacteria was analyzed. The result showed that the ratio of CA produced bacteria is 11.6% in a typical cyanobacterial bloom from Lake Taihu. An attached bacterium strain P201, which produced large amount of extracellular carbonic anhydrase, was isolated from M. aeruginosa assemblage. It was identified as a strain of Pseudomonas fluorescence by its ITS molecular characteristic. Effects of P201 on the growth of M. aeruginosa in different HCO3- concentrations were investigated. The results showed that no matter in the higher or the lower HCO3- culture medium, P201 could promote the growth of M. aeruginosa, which suggested that CA produced attached bacteria could enhance the growth of M. aeruginosa by regulating the ambient carbon environment.
Key words:  Microcystis aeruginosa  Pseudomonas fluorescence  attached bacteria  carbonic anhydrase
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