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引用本文:赵婕,李建宏,管章玲,许玲,潘澄,李朋富.一株产碱性磷酸酶附生菌对微囊藻生长的影响.湖泊科学,2011,23(1):49-55. DOI:10.18307/2011.0108
ZHAO Jie,LI Jianhong,GUAN Zhangling,XU Ling,PAN Cheng,LI Pengfu.Effect of an attached bacterium of alkaline phosphatase producting on the growth of Microcystis aeruginosa. J. Lake Sci.2011,23(1):49-55. DOI:10.18307/2011.0108
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一株产碱性磷酸酶附生菌对微囊藻生长的影响
赵婕1, 李建宏1, 管章玲1, 许玲1, 潘澄1, 李朋富2
1.南京师范大学生命科学学院, 江苏省生物技术与生物多样性重点实验室, 南京 210046;2.南京大学生命科学学院, 南京 210093
摘要:
为研究产碱性磷酸酶(ALP)细菌对微囊藻(Microcystis aeruginosa)从有机磷中获得磷营养的影响, 从微囊藻群体中分离获得一株产ALP附生菌Y6, 通过ITS分子鉴定该菌属赤杆菌属(Erythrocter).探讨该菌在有机磷条件下对微囊藻生长及生理代谢的影响, 结果表明:(1)细菌发酵液中ALP活性与Y6菌的浓度呈正相关, 在培养初期的延滞期即开始产生ALP;(2)在有机磷源条件下Y6的加入对微囊藻生长有促进作用;(3)有机磷源条件下, Y6有助于维持群体微囊藻的上浮率和较大群体, Y6的加入也有维持藻细胞叶绿索荧光强度的作用.本研究的结果表明, 产ALP附生菌有助于微囊藻利用水体中有机磷, 有利于水华形成。
关键词:  微囊藻  藻上附生菌  碱性磷酸酶  Chl.a荧光强度  水华
DOI:10.18307/2011.0108
分类号:
基金项目:江苏省科技厅社会发展项目(BS200765)和国家重点基础研究发展计划"973"项目(2008CB418004)联合资助
Effect of an attached bacterium of alkaline phosphatase producting on the growth of Microcystis aeruginosa
ZHAO Jie1, LI Jianhong1, GUAN Zhangling1, XU Ling1, PAN Cheng1, LI Pengfu2
1.College of Life Sciences, Jiangsu Key Laboratory for Biotechnology and Biodiversity, Nanjing Normal University, Nanjing 210046, P.R.China;2.School of Life Sciences, Nanjing University, Nanjing 210093, P.R.China
Abstract:
To study the effect of alkaline phosphatase(ALP) bacteria on Microcysfis aeruginosa obtaining phosphorus from organic phosphorus compounds, an attached bacterium strain Y6 which produced ALP was isolated from M.aeruginosa colonies.It was identified as a strain of Erythrobacter by its ITS molecular characteristic.The effects of the bacterium on the growth and physiology of M.aeruginosa in the organic phosphorus condition investigated in this paper.The results show that:(1) ALP activity in the culture broth was positively related Y6 concentration, initial lag phase of incubation began to produce ALP;(2) Under the condition of organic phosphorus, Y6 could promote the growth of M.aeruginosa;(3) Y6 could maintain floating rate and bigger colonies of colonial M.aeruginosa, and hold chlorophyll fluorescence of algal cells in the organic phosphorus medium.This research suggested that ALP producing attached bacteria could help M.aeruginosa using organic phosphorus and forming blooms.
Key words:  Microcystis aeruginosa  attached bacteria  alkaline phosphatase  Chl.a fluorescence intensity  water bloom
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