| 引用本文: | 周丽华,陈士超,邓志瑞,陈沁,郑乐平,刘志学.太湖沉积物中的可培养细菌:Ⅰ.细菌多样性初步分析.湖泊科学,2009,21(1):27-35. DOI:10.18307/2009.0104 |
| ZHOU Lihua,CHEN Shichao,DENG Zhirui,CHEN Qin,ZHENG Leping,LIU Zhixue.Culturable bacteria in the sediment of Lake Taihu (I):primary analysis of bacterial diversity. J. Lake Sci.2009,21(1):27-35. DOI:10.18307/2009.0104 |
|
| 摘要: |
| 采用牛肉膏培养基培养,从不同深度的太湖沉积物中共分离得到603个菌落形态不同的菌株,通过数值分析比较了40个形态表征性状,以不加权平均连锁聚类(UPGMA)的方式进行簇群归类,在0.75水平上可分成4个聚类群和51个亚群.从51个亚群中各随机选取一个菌株,测定其16SrDNA部分序列(约540bp)并据此进行分析,表明这些菌株分别属于Firmicutes、Actinobacteria、γ-Proteobacteria这三大类群,与数据库中已知的芽孢杆菌属(Bacillus)、类芽孢杆菌属(Paenibacillus)、微球菌属(Micrococcus)、节杆菌属(Arthrobacter)、迪茨菌属(Dietzia)和假单胞菌属(Pseudomonas)细菌具有很高的相似性.其中迪茨菌属在国内淡水湖泊沉积物中为首次报道. |
| 关键词: 太湖 沉积物 可培养细菌 数值分类 多样性 |
| DOI：10.18307/2009.0104 |
| 分类号: |
| 基金项目:国家自然科学基金项目(40573063)资助． |
|
| Culturable bacteria in the sediment of Lake Taihu (I):primary analysis of bacterial diversity |
|
ZHOU Lihua1, CHEN Shichao2, DENG Zhirui3, CHEN Qin3, ZHENG Leping1, LIU Zhixue2
|
|
1.School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, P. R. China;2.School of Life Science and Technology, Tongji University, Shanghai 200092, P. R. China;3.School of Life Science, Shanghai University, Shanghai 200444, P. R. China
|
| Abstract: |
| The sediment samples collected from the different sites of the Lake Taihu, 2006 were cultured on traditional nutrient medium. 603 bacteria strains were separated with 40 different morphological features, and were grouped into 4 clusters and 51 subclusters at the level of 0.75 with numerical classified based on the unweighted pair group method with arithmetic averages (UPGMA) algorithm. Based on the partial sequences of 16SrDNA (540bp) analysis, the bacteria strains sample chosen from each subclusters randomly were ranked into Firmicutes, Actinobacteria and γ-Proteobacteria three groups, and closely related to Bacillus, Paenibacillus, Micrococcus, Arthrobacter, Dietzia and Pseudomonas genera. The dietzia genera was reported in the sediment of freshwater lake in China for the first time. |
| Key words: Lake Taihu sediment culturable bacteria numerical taxonomy diversity |
