投稿中心

审稿中心

编辑中心

期刊出版

网站地图

友情链接

引用本文:周丽华,陈士超,邓志瑞,陈沁,郑乐平,刘志学.太湖沉积物中的可培养细菌:Ⅰ.细菌多样性初步分析.湖泊科学,2009,21(1):27-35. DOI:10.18307/2009.0104
ZHOU Lihua,CHEN Shichao,DENG Zhirui,CHEN Qin,ZHENG Leping,LIU Zhixue.Culturable bacteria in the sediment of Lake Taihu (I):primary analysis of bacterial diversity. J. Lake Sci.2009,21(1):27-35. DOI:10.18307/2009.0104
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 4858次   下载 3378 本文二维码信息
码上扫一扫!
分享到: 微信 更多
太湖沉积物中的可培养细菌:Ⅰ.细菌多样性初步分析
周丽华1, 陈士超2, 邓志瑞3, 陈沁3, 郑乐平1, 刘志学2
1.上海大学环境与化学工程学院, 上海 200444;2.同济大学生命科学与技术学院, 上海 200092;3.上海大学生命科学学院, 上海 200444
摘要:
采用牛肉膏培养基培养,从不同深度的太湖沉积物中共分离得到603个菌落形态不同的菌株,通过数值分析比较了40个形态表征性状,以不加权平均连锁聚类(UPGMA)的方式进行簇群归类,在0.75水平上可分成4个聚类群和51个亚群.从51个亚群中各随机选取一个菌株,测定其16SrDNA部分序列(约540bp)并据此进行分析,表明这些菌株分别属于Firmicutes、Actinobacteria、γ-Proteobacteria这三大类群,与数据库中已知的芽孢杆菌属(Bacillus)、类芽孢杆菌属(Paenibacillus)、微球菌属(Micrococcus)、节杆菌属(Arthrobacter)、迪茨菌属(Dietzia)和假单胞菌属(Pseudomonas)细菌具有很高的相似性.其中迪茨菌属在国内淡水湖泊沉积物中为首次报道.
关键词:  太湖  沉积物  可培养细菌  数值分类  多样性
DOI:10.18307/2009.0104
分类号:
基金项目:国家自然科学基金项目(40573063)资助.
Culturable bacteria in the sediment of Lake Taihu (I):primary analysis of bacterial diversity
ZHOU Lihua1, CHEN Shichao2, DENG Zhirui3, CHEN Qin3, ZHENG Leping1, LIU Zhixue2
1.School of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, P. R. China;2.School of Life Science and Technology, Tongji University, Shanghai 200092, P. R. China;3.School of Life Science, Shanghai University, Shanghai 200444, P. R. China
Abstract:
The sediment samples collected from the different sites of the Lake Taihu, 2006 were cultured on traditional nutrient medium. 603 bacteria strains were separated with 40 different morphological features, and were grouped into 4 clusters and 51 subclusters at the level of 0.75 with numerical classified based on the unweighted pair group method with arithmetic averages (UPGMA) algorithm. Based on the partial sequences of 16SrDNA (540bp) analysis, the bacteria strains sample chosen from each subclusters randomly were ranked into Firmicutes, Actinobacteria and γ-Proteobacteria three groups, and closely related to Bacillus, Paenibacillus, Micrococcus, Arthrobacter, Dietzia and Pseudomonas genera. The dietzia genera was reported in the sediment of freshwater lake in China for the first time.
Key words:  Lake Taihu  sediment  culturable bacteria  numerical taxonomy  diversity
分享按钮