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引用本文:杨州,孔繁翔,史小丽,张民,曹焕生.棕鞭毛虫牧食作用对铜绿微囊藻形态和生理特性的影响.湖泊科学,2008,20(4):403-408. DOI:10.18307/2008.0401
YANG Zhou,KONG Fan-xiang,SHI Xiao-li,ZHANG Min,CAO Huan-sheng.Grazing effect of Ochromonas sp. on morphological and physiological characteristics of Microcystis aeruginosa. J. Lake Sci.2008,20(4):403-408. DOI:10.18307/2008.0401
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棕鞭毛虫牧食作用对铜绿微囊藻形态和生理特性的影响
杨州1,2, 孔繁翔2, 史小丽2, 张民2, 曹焕生2
1.南京师范大学生命科学学院, 南京 210046;2.中国科学院南京地理与湖泊研究所, 南京 210008
摘要:
在纯培养的铜绿微囊藻种群中添加有效牧食者棕鞭毛虫,进行为期9d的实验,用流式细胞仪检测棕鞭毛虫牧食作用对铜绿微囊藻的影响.结果表明棕鞭毛虫的牧食导致微囊藻种群迅速下降,同时微囊藻种群出现了群体形成现象.棕鞭毛虫直接牧食作用诱发微囊藻形成群体后,能在一定程度上能防御棕鞭毛虫的进一步牧食,使得种群得以延续.在棕鞭毛虫的牧食作用下,以酯酶活性和叶绿素荧光强度为代表的微囊藻细胞活性在实验后期显著增强.而牧食处理后细胞尺寸有所变小,这可能是伴随着微囊藻诱发性群体形成过程中一种生态对策的调整,保持较小的个体有利于种群的迅速增殖.
关键词:  棕鞭毛虫  铜绿微囊藻  群体形成  酯酶活性  叶绿素  细胞尺寸
DOI:10.18307/2008.0401
分类号:
基金项目:国家自然科学基金项目(30670404,30400062);江苏省自然科学基金项目(BK2007743);国家重点基础研究发展计划项目(2002CB412305)联合资助
Grazing effect of Ochromonas sp. on morphological and physiological characteristics of Microcystis aeruginosa
YANG Zhou1,2, KONG Fan-xiang2, SHI Xiao-li2, ZHANG Min2, CAO Huan-sheng2
1.School of Biological Sciences, Nanjing Normal University, Nanjing 210046, P.R.China;2.Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P.R.China
Abstract:
In laboratory culture Microcystis aeruginosa occurs mainly as single cells but under natural conditions, it is normally in the colonial morph. This phenomenon suggests that some factors may be responsible for the occurrence of colonies in lakes. In the present experiment we investigated the grazing effect of flagellate Ochromonas sp. on physiological changes in M. aeruginosa using flow cytometry. The experiment was run in triplicate for 9 days at 25℃ in photoperiod of 12L:12D. Results showed that M. aeruginosa population exposed to flagellate grazing decreased sharply and some single cells formed colonies, most of which were made up of several or dozens of cells. Colony formation can deter flagellate from further grazing on a certain extent. Esterase activity and chlorophyll fluorescence of M. aeruginosa cells under flagellate grazing were significantly higher than those of unicells in the control, whereas the cell size of M. aeruginosa became smaller under intense flagellate grazing. The enhanced esterase activity may contribute to colony formation. The fact that cell size of M. aeruginosa under flagellate grazing became smaller may indicate that M. aeruginosa adopt a kind of ecological strategy to grow rapidly under intensive grazing.
Key words:  Flagellate Ochromonas sp.  Microcystis aeruginosa  colony formation  esterase activity  chlorophyll  cell size
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