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引用本文:蒋金辉,周长芳,安树青,关保华,蔡颖.工具种轮叶黑藻的组织培养与快速繁殖.湖泊科学,2008,20(2):215-220. DOI:10.18307/2008.0213
JIANG Jinhui,ZHOU Changfang,AN Shuqing,GUAN Baohua,CAI Ying.Vast propagation of submerged tool species Hydrilla verticillata with tissue culture method. J. Lake Sci.2008,20(2):215-220. DOI:10.18307/2008.0213
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工具种轮叶黑藻的组织培养与快速繁殖
蒋金辉,周长芳,安树青,关保华,蔡颖
1.南京大学生命科学学院, 湿地生态研究所, 南京 210093;2.中国科学院南京地理与湖泊研究所, 南京 210008
摘要:
利用组织培养技术对工具种轮叶黑藻进行快繁研究,建立无菌体系后,在0.5和1倍MS培养液中添加0.5-2mg/LBA及0.1-0.2mg/L IAA进行芽诱导:在0.25、0.5和1倍MS培养液中添加0.1-0.2mg/L IAA进行根诱导.结果发现,1倍MS+ 1mg/L BA+0.1mg/L IAA培养基获得最大芽数及芽生物量,最适合芽诱导;0.5倍MS+0.1mg/LIAA或1倍MS+0.2mg/L IAA培养基最能促进根的发展.芽增殖使轮叶黑藻繁殖效率达每季度1800倍以上;再经根诱导,快速形成完整植株,提高定居能力.在室外水体中以两种密度(100株/m~2,320株/m~2),,三种底质(沙,泥,泥+沙1. 1 V/V))上进行移栽,成活率100%.21d后,高密度群落覆盖度大于70%.表明该技术不仅可在短期内获得大量种苗,且种质优,为沉水植被恢复工程奠定关键的基础.
关键词:  组织培养  沉水植被  大规模扩繁  湿地恢复
DOI:10.18307/2008.0213
分类号:
基金项目:科学技术部太湖“863”专项(2002AA601012-06);镇江“863”专项(2003AA06011000-04)联合资助
Vast propagation of submerged tool species Hydrilla verticillata with tissue culture method
JIANG Jinhui1,2,3, ZHOU Changfang1,2, AN Shuqing1,2, GUAN Baohua2, CAI Ying2
1.State Key Laboratory of Pollution Control and Resource Uses, School of Life Science, Nanjing University, Nanjing 210093, P. R. China;2.Institute of Wetland Ecology, School of Life Science, Nanjing University, Nanjing 210093, P. R. China;3.Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China
Abstract:
The vast propagation of Hydrilla verticillata was studied based on the tissue culture technique. After aseptic system wasestablished, half and full strength of Murashige and Skoog-based liquid media(MS), in addition with 0.5-2 mg/L 6-Benzylaminopurine(BA) and 0.1-0.2 mg/L indoleacetic acid (IAA), were used for shoot induction. Meanwhile, quarter, half and full strength of MS plus0.1-0.2 mg/L IAA were useded for root induction. The results showed that the combination of full strength of MS plus 1 mg/L BAand 0.1 mg/L IAA was the best medium for shoot induction because the plants on such combined condition had more shoot numberand shoot biomass than those in the other mixtures. Half strength of MS plus 0.1 mg/L IAA or full strength MS plus 0.2 mg/L IAAwas the most proper media for root induction, and they can greatly promote the development of roots. The propagation was greatlyenhanced with the shoot induction in the optimal environment, amounting to 1800 times per quarter. The root induction made theseshoots grow into whole plants quickly, which enhanced the colonization ability of propagules at new habitat. In the outdoorexperiment, all plants survived at two densities (100 plants/m2, 320 plants/m2) on three sediment types (sand, clay, sand+ clay1. 1V/V)). After 21 days, the population coverage was more than 70%. The experiments demonstrated that tissue culture was an effectivemethod of propagation of tool species to get the vast number of seedlings, and ensures the quality of the plants, and finally establishesa key basis for restoration engineering of submerged vegetation.
Key words:  Tissue culture  submerged vegetation  vast propagation  wetland restoration
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