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引用本文:张余霞,张玲,高兴,王立新,陆长梅,吴国荣.水稻(Oryza sativa L.)秸秆浸提液对铜绿微囊藻(Microcysti aeruginosa)的抑制作用.湖泊科学,2007,19(4):479-484. DOI:10.18307/2007.0418
ZHANG Yuxia,ZHANG Ling,GAO Xing,WANG Lixing,LU Changmei,WU Guorong.The inhibition of aqueous extract from Oryza sativa straw on growth of Microcystis aeruginosa. J. Lake Sci.2007,19(4):479-484. DOI:10.18307/2007.0418
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水稻(Oryza sativa L.)秸秆浸提液对铜绿微囊藻(Microcysti aeruginosa)的抑制作用
张余霞1,2, 张玲1, 高兴1, 王立新1,3, 陆长梅1, 吴国荣1
1.南京师范大学生命科学学院, 南京 210097;2.盐城工学院, 盐城 224003;3.常熟理工大学, 常熟 215500
摘要:
用刚收割的水稻(Oryza sativa L.)秸秆浸提液代替水配制培养基培养铜绿微囊藻(Microcysti aeruginosa), 追踪测定微囊藻的生物量、叶绿素a含量、光合速率、呼吸速率、膜透性、超氧物歧化酶(SOD)活性、光合膜自发荧光强度等相关生理生化指标的变化, 研究水稻秸秆浸液对铜绿微囊藻的抑制作用。结果表明, 质量比≥1/100(水稻秸秆/水)的水稻秸秆浸提液对微囊藻的生长有明显的抑制作用, 表现如下:藻细胞生物量在实验过程中逐日降低, 藻细胞叶绿素a含量和光合速率急剧下降, 呼吸速率和超氧物歧化酶(SOD)活性呈先升高后下降的趋势, 膜透性迅速升高, 细胞光合膜自发荧光强度显著衰减。用不同的有机溶剂对该浸提液进行萃取, 浓缩后用滤纸片法在固体培养基上做抑藻实验, 乙醚和乙酸乙酯萃取液能明显看到抑藻圈, 证实其中含有抑制物质。
关键词:  水稻秸秆水浸提液  铜绿微囊藻  抑制作用  抑制物质
DOI:10.18307/2007.0418
分类号:
基金项目:国家自然科学基金项目(30370083);教育部科学技术研究项目(01043)联合资助
The inhibition of aqueous extract from Oryza sativa straw on growth of Microcystis aeruginosa
ZHANG Yuxia1,2, ZHANG Ling1, GAO Xing1, WANG Lixing1,3, LU Changmei1, WU Guorong1
1.College of Life Science, Nanjing Normal University, Nanjing 210097 P. R. China;2.Yancheng College, Yancheng 224003 P. R. China;3.Changshu College, Changshu 215500 P. R. China
Abstract:
Microcystis aeruginosa was co-cultivated with aqueous extract from fresh reaped Oryza sativa L. straw and the inhibiting effect of this aqueous extract on M. aeruginosa was studied in this paper. Results indicated that the growth of M. aeruginosa was obviously inhibited when the rate of quality was over 1/100(rice straw/water). Chlorophyll a content, photosynthetic rate and the intensity of spontaneous fluorescence decreased continuously as the treatment went on ; respiratory rate and SOD activity decreased evidently at the first 3 days of treatment and then increased ; membrane penetrability increased at the first 5 days of treatment and then decreased slowly. Some substances were extracted from the aqueous extract by different organic solvents (ether, ethyl acetate and petroleum e-ther 30-60℃), respectively. Inhibiting effect could be observed with filtering paper methods on solid medium when the substances were extracted by ether, ethyl acetate. This suggested that some inhibitive factors might exist in these substances.
Key words:  aqueous extraction from rice straw  M. aeruginosa  inhibiting effect  inhibitors
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