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引用本文:邓文娜,余育和,冯伟松,颜庆云,宋晓红,刘志新.三峡库区浮游生物群落结构与DNA多态性比较分析.湖泊科学,2007,19(3):333-339. DOI:10.18307/2007.0316
DENG Wenna,YU Yuhe,FENG Weisong,YAN Qingyun,SONG Xiaohong,LIU Zhixin.Comparative analysis between species composition and DNA polymorphism of plankton community in the Three-Gorges Reservoir region. J. Lake Sci.2007,19(3):333-339. DOI:10.18307/2007.0316
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三峡库区浮游生物群落结构与DNA多态性比较分析
邓文娜1,2, 余育和1, 冯伟松1, 颜庆云1,2, 宋晓红1,2, 刘志新1,2
1.中国科学院水生生物研究所, 武汉 430072;2.中国科学院研究生院, 北京 100039
摘要:
以三峡库区所设A、B、C、D、E、F和G等7个站点为研究区域, 利用RAPD技术就其浮游生物群落遗传多样性与物种多样性的关系进行了探索性研究。共鉴定出浮游生物41种, 其中藻类4种, 原生动物10种, 轮虫类19种, 枝角类4种, 桡足类4种。物种组成相似性最高的是B站和C站, 最低的是A站和F站。而站点间浮游生物群落遗传距离分析表明:相距最近的是C站与D站, 最远的为D站与F站。树状聚类图也显示:各站点物种组成聚为一类;而DNA多态性RAPD最先聚在一起的是B与F, 它们的对应枝由A、C、D、E构成, G单独成为一枝。研究表明, 所发现的物种组成与所揭示的遗传多样性并未完全吻合。这或许是物种信息的缺失(如藻类与细菌), 或许是RAPD技术的缺陷, 或许两者所致。因此, 本研究积累了三峡库区浮游生物群落遗传多样性的一些背景资料, 而要阐释浮游生物群落遗传多样性与物种多样性之间的相互关系则有赖于应用灵敏的、重复性好的, 且能分别分析原核和真核生物的DNA分子标记技术。
关键词:  三峡库区  浮游生物  RAPD  遗传多样性  物种多样性
DOI:10.18307/2007.0316
分类号:
基金项目:国家自然科学基金项目(30570240,30490232);国家"973"计划(2002CB412308)联合资助
Comparative analysis between species composition and DNA polymorphism of plankton community in the Three-Gorges Reservoir region
DENG Wenna1,2, YU Yuhe1, FENG Weisong1, YAN Qingyun1,2, SONG Xiaohong1,2, LIU Zhixin1,2
1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China;2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, P. R. China
Abstract:
Recently, molecular ecology has been appearing to increasing attention, many kinds of molecular methods were applied in this field.In addition, various experts have been interesting in the Three-Gorges Reservoir region (TGRR) and even the whole Yangtze drainage basin after the beginning of dam engineering.However, few studies were focused on the molecular ecology of TGRR.In order to explore the relationship between genetic diversity and species diversity, the whole DNA of plankton communities from seven samples locations in the TGRR was analyzed by random amplified polymorphic DNA (RAPD), and species of which were identified as well.The 7 locations can be clustered into 3 groups by genetic diversity.Station A, C, D and E cluster as one group, station B and F cluster as another group, and station G is different from all of others.While these stations still as a whole after analyzing by RAPDistancel.04.Also, 41 species were found in all, which contains 4 algae, 10 protozoa, 19 rotifer, 4 cladocera and copepoda respectively.Meanwhile, the nearest genetic distance is only 0.2586 which occurred between station D and C, and the opposite one lies between D and F which is 0.4758.But species diversity analysis displays that station B and C have the nearest distance;correspondingly, A and F have the furthest one.In other words, the result of genetic diversity is not consistent with that of species diversity.Therefore, more information has been obtained from genetic diversity in river, which applied new information for establishing the relationship between genetic diversity and species diversity of plankton.And more molecular biological methods should be applied, such as that more sensitive as well as good replied approaches could analyze pronucleus and eukaryon re-spectively.
Key words:  Three-Gorges Reservoir Region(TGRR)  plankton  RAPD  genetic diversity  species diversity
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