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引用本文:程起群,温俊娥,王云龙,韩金娣,卢大儒.刀鲚与湖鲚线粒体细胞色素b基因片段多态性及遗传关系.湖泊科学,2006,18(4):425-430. DOI:10.18307/2006.0416
CHENG Qiqun,WEN June,WANG Yunlong,HAN Jindi,LU Daru.Genetic diversity and genetic differentiation between Coilia ectenes and Coilia ectenes taihuensis inferred from cytochrome b gene segment sequence of mitochondrial DNA. J. Lake Sci.2006,18(4):425-430. DOI:10.18307/2006.0416
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刀鲚与湖鲚线粒体细胞色素b基因片段多态性及遗传关系
程起群1,2, 温俊娥3, 王云龙1, 韩金娣1, 卢大儒2
1.农业部海洋与河口渔业重点实验室, 中国水产科学研究院东海水产研究所, 上海 200090;2.复旦大学遗传学研究所, 遗传工程国家重点实验室, 上海 200433;3.上海复生生物工程研究所, 上海 200433
摘要:
使用线粒体细胞色素6基因片段序列为分子标记,分析了刀鲚和湖鲚的遗传多态性及遗传关系.在两个群体中共检测到5种不同的单倍型.刀鲚和湖鲚的单倍型多样性指数分别为0.9000和0.7000;核苷酸多样性指数分别为0.3763%和0.4301%.分子方差分析显示两者遗传分化的水平很低,群体间的差异对总的变异没有贡献.所以认为它们之间的差异尚未达到亚种水平,这一结果支持湖鲚是刀鲚的一个地理种群的观点.
关键词:  刀鲚  湖鲚  细胞色素b  遗传多样性  遗传差异  进化
DOI:10.18307/2006.0416
分类号:
基金项目:中国水产科学研究院青年基金项目(2003-青-4);农业部海洋与河口渔业重点实验室开放课题(开-1-04-14);农业部海洋渔业资源可持续利用重点实验室开放课题(实开2005-06)共同资助
Genetic diversity and genetic differentiation between Coilia ectenes and Coilia ectenes taihuensis inferred from cytochrome b gene segment sequence of mitochondrial DNA
CHENG Qiqun1,2, WEN June3, WANG Yunlong1, HAN Jindi1, LU Daru2
1.Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, P. R. China;2.State Key Laboratory of Genetic engineering, Institute of Genetics, Fudan University, Shanghai 200433, P. R. China;3.Shanghai Fusheng Institute of Bio-engineering, Shanghai 200433,P. R. China
Abstract:
Genetic diversity and genetic differentiation between Coilia ectenes and Coilia ectenes taihuensis have been investigated at the mtDNA level. Sequences analysis of cytochrome b (cytb) gene segments amplified by PCR was used. Five different haplotypes were detected between the populations studied. The haplotype diversity of C. ectenes and C. e. taihuensis were 0.9000 and 0.7000,respectively. The nucleotide diversity of C. ectenes and C. e. taihuensis were 0.3763% and 0.4301%, respectively. An hierarchical analysis of molecular variance (AMO-VA) showed that very low levels of genetic partitioning, with 0.00% of the total variation attributed to between population differences, and lack of genetic structure. Our results supported that C. e. taihuensis is a geographical population of C. ectenes; their difference has not risen to subspecies level.
Key words:  Coilia ectenes  Coilia ectenes taihuensis  cytochrome b  genetic diversity  genetic differentiation  evolution
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