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引用本文:张文静,余育和,沈韫芬,颜庆云.七株眼虫基于微卫星DNA指纹图谱的区分和关系分析.湖泊科学,2006,18(3):285-292. DOI:10.18307/2006.0315
ZHANG Wenjing,YU Yuhe,SHEN Yunfen,YAN Qingyun.Separation and relationships of seven Euglena strains based on microsatellite DNA finger-printing. J. Lake Sci.2006,18(3):285-292. DOI:10.18307/2006.0315
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七株眼虫基于微卫星DNA指纹图谱的区分和关系分析
张文静1,2, 余育和1, 沈韫芬1, 颜庆云1,2
1.中国科学院水生生物研究所淡水生态与生物技术国家重点实验室原生动物分类与生态组, 武汉 430072;2.中国科学院研究生院, 北京 100039
摘要:
尽管以前对眼虫进行过大量的形态发育研究和基于核糖体RNA基因的系统发育分析,但对于株系之间的关系仍然知之甚少.因其形态特征有限并且易变,很难鉴定眼虫的相似种和同种内不同的株.作者利用微卫星DNA指纹图谱,在七株眼虫中扩增了七个微卫星DNA位点,成功扩增的六个微卫星引物都得到了四到八个条带.从微卫星DNA指纹图谱计算得到的相似性系数范围从0.000到0.957.根据相似性系数得到的树状结构,七株眼虫在距离为0.9346处分为三支:E.mutabilis,E.intermedia和E.gracilis.其中,五株E.gracilis分为两组:来自日本的和美国的.不同地区的株得到不同的基因型,并初步分析了它们之间的关系.研究表明七株眼虫根据微卫星DNA指纹图谱被明显区分开.微卫星DNA指纹图谱具有很高的分辨率,是鉴定和区分原生动物相似种和同种内不同株的一种有用的新方法.
关键词:  眼虫  微卫星DNA  原生动物  相似性关系  株的区分
DOI:10.18307/2006.0315
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Separation and relationships of seven Euglena strains based on microsatellite DNA finger-printing
ZHANG Wenjing1,2, YU Yuhe1, SHEN Yunfen1, YAN Qingyun1,2
1.State Key Laboratory of Freshwater Ecology and Biotechnology, Laboratory of Taxonomy and Ecology of Protozoa, Institute of Hydrobiology, Chinese Academy of Sciences, 430072 Wuhan, P. R. China;2.Graduate School of the Chinese Academy of Sciences, 100039 Beijing, P. R. China
Abstract:
The genetic relationships among Euglena strains remain poorly understood, despite a rich history of morphological examination and an extensive phylogenetic analysis of the ribosomal RNA genes. It is difficult to identify related species of the genus Euglena and to separate different strains of the same species because the morphological characters are variable and limited. We address this question using microsatellite DNA fingerprinting. Seven microsatellite loci were amplified from 7 Euglena strains. A total of 4-8 bands were successfully amplified for each of 6microsatellite primer pairs. Microsatellite DNA fingerprinting and similarity values ranging from 0.000 to 0.957 a-mong the seven strains were obtained, which were used to construct a similarity tree among them. According to the tree, at the linkage distance of 0.9346 seven strains were separated into three clusters: E. mutabilis, E. intermedia and E. gracilis consisting of Japanese and USA groups. Further, in the cluster E. gracilis, different genotypes were recognized for the strains from different geographical origins. The relationships among species and strains were analyzed preliminarily. We demonstrates that the seven strains of Euglena can be clearly separated by their microsatellite DNA fingerprinting patterns. The microsatellite DNA fingerprinting has a high resolution and is a new useful method to identify and separate similar species and intraspecific strains in free-living protozoa.
Key words:  Euglena  mierosatelite DNA:protozoa  similarity relationships  stirans separalion
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