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引用本文:宦海琳,韩岚,李建宏,翁永萍.五株微囊藻毒素降解菌的分离与鉴定.湖泊科学,2006,18(2):184-188. DOI:10.18307/2006.0213
HUAN Hailin,HAN Lan,LI Jianhong,WENG Yongping.Isolation and identification of five microcystin degrading bacterial strains. J. Lake Sci.2006,18(2):184-188. DOI:10.18307/2006.0213
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五株微囊藻毒素降解菌的分离与鉴定
宦海琳, 韩岚, 李建宏, 翁永萍
南京师范大学,江苏省生物多样性与生物技术重点实验室 南京 210097
摘要:
有毒蓝藻产生的蓝藻毒素导致地表水的污染已是全球普遍的现象,最常见的毒素是能导致肝癌的肝毒素微囊藻毒素(MC),迄今为止尚无适用的MC驱除技术,微生物降解MC可能是-条有效的途径.本研究以铜绿微囊藻(Microcystis aeruginosa PCC 7806)所产生的MC为底物,从多个形成微囊藻水华的水体中分离获得能显著降解MC的细菌18株,相对降解能力从18.4%到48.2%.对其中5株MC相对降解能力较强的菌,通过形态特征和生理生化特征分析,鉴定出5株菌分别为:不动细菌属(Acinetobacter)、肠杆菌属(Enterobacter)、微杆菌属(Microbacterium)、芽孢杆菌属(Bacillus)和弗拉特氏菌属(Frateuria),均不同于已报道的属种.
关键词:  微囊藻毒素  细菌降解  水华  蓝藻毒素
DOI:10.18307/2006.0213
分类号:
基金项目:教育部归国留学人员基金(2003);江苏省教委基金(03KJB180113)联合资助
Isolation and identification of five microcystin degrading bacterial strains
HUAN Hailin, HAN Lan, LI Jianhong, WENG Yongping
Nanjing Normal University, Key Lab of Biodiversity and Biotechnology of Jiangsu Province, Nanjing 210097 P. R. China
Abstract:
Cyanotoxins produced by cyanobacteria have caused pollutions in land surface water around the world.The most common toxin is microcystin(MC),a cancer inducing hepatoxin. No effective technology could be used to remove it up to now. Microbiulogical degradation should be a suitable way. In this study, we used BG-ll rnedium to culture Mi.crocystis nerngiraosa PCC 7806 in the library.MC was extracted by 70% methanol, then concenteafed and purified by C}fl solid-phase extraction collum. The relative contents of MC-LR and MC-RR were 68.2% and 9.3%, respectively, on the basis of high performance liquid chromatography(HPLC) analysis. We used this MG as seleraive substrate to isolate MC degradable bacaeria. From cyanobacterial bloom forming water body such as Xuanwu Lake,Qinhuai Rever, we got eighteen bacterial strains, which could degrade MC-LR obviously. The relafive efficiencies were from 18.4%to 48.2%.Five more effective strains of them were identified as rlcinetaLncter,Enterahacter, Microhacteriu.m, Bacillus and, Fratetcria, respectively by there morphology and biochemical characteristics. These strains were all different from the published ones up to now.
Key words:  Microcystin  bacterial degradation  cyanobacterial bloorn  cyanotoxin
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