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引用本文:吴国荣,陆长梅,陶明煊,周长芳,顾龚平,魏锦城.百草枯和H2O2预处理提高盐泽螺旋藻对铅的耐受性.湖泊科学,2000,12(3):240-246. DOI:10.18307/2000.0308
WU Guorong,LU Changmei,TAO Mingxuan,ZHOU Changfang,GU Gongping,WEI Jincheng.Enhancement of Spirulina subsalsa Tolerance to Pb by Pretreatment of Paraquat and H2O2. J. Lake Sci.2000,12(3):240-246. DOI:10.18307/2000.0308
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百草枯和H2O2预处理提高盐泽螺旋藻对铅的耐受性
吴国荣, 陆长梅, 陶明煊, 周长芳, 顾龚平, 魏锦城
南京师范大学生命科学学院, 南京210097
摘要:
用 10μmol·L-1的百草枯 (Paraquat)和 2 mmol·L-1的 H2O2 对盐泽螺旋藻(Spirulina subsalsa)作预处理,以诱导提高藻细胞内超氧物歧化酶 (SOD),过氧化氢酶 (CAT)和过氧化物酶 (POD)的活性,随后用 4 0 mg·L-1的 Pb2+进行胁迫试验.实验结果表明,预处理组的藻细胞生长量和光合速率的下降幅度明显较未预处理组小;预处理组的 SOD,CAT活性虽有衰减,但与未预处理组相比仍保持较高的水平,有效地抑制了藻细胞内 O2 ·和丙二醛 (MDA)的积累;预处理组 POD活性的升幅介于未预处理组和正常生长的藻细胞之间.可以推断,诱导提高藻细胞抗氧化的能力,有利于减轻 Pb2+对藻细胞的毒害.
关键词:  盐泽螺旋藻  百草枯  H2O2  抗氧化酶  Pb2+胁迫
DOI:10.18307/2000.0308
分类号:
基金项目:江苏省自然科学基金资助项目(1998SWXOSZ0001).
Enhancement of Spirulina subsalsa Tolerance to Pb by Pretreatment of Paraquat and H2O2
WU Guorong, LU Changmei, TAO Mingxuan, ZHOU Changfang, GU Gongping, WEI Jincheng
College of Life Science, Nanjing Normal University, Nanjing 210097, China
Abstract:
In this research Spirulina subsala was firstly pretreated by paraquat (10μmol° L-1) and H2O2 (2mmol° L-1) to induce its antioxidant enzymes, then were treated under Pb2+ stress (40mg° L-1).The results showed that the decreases of both grow th yield and photosynthesic rate of pretreated Spirulina subsalsa were less than those of the non-pretreated.Although the activities of SOD and CAT were also decreased, they were higher than those of the non-pretrea ted, and they inhibited the accumunation of O2 and M DA effectively.The range of POD increase was between those of the non-pretreated and the control.It could be concluded that induction could increase the antioxidant capacity, and could help Spirulina subsala resist Pb2+'s toxity.
Key words:  Spirulina subsalsa  paraquat  H2O2  antioxidant enzymes  Pb2+ stress
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