| 引用本文: | 周长芳,吴国荣,陆长梅,陈国祥,魏锦城.铅污染对钝顶螺旋藻生长及某些生理性状的影响.湖泊科学,1999,11(2):135-140. DOI:10.18307/1999.0208 |
| ZHOU Chang-fang,WU Guo-rong,LU Chang-mei,CHEN Guo-xiang,WEI Jin-cheng.Effect of Pb2+ on the Growth of Spirulina platensis and Its Physiological Characters. J. Lake Sci.1999,11(2):135-140. DOI:10.18307/1999.0208 |
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| 摘要: |
| 通过在Zarrouk培养基中加入不同浓度的Pb2+研究水环境污染对钝顶螺旋藻(Spiriulina platensis)生长、藻蓝蛋白和光合作用、膜脂过氧化反应及以超氧物歧化酶(SOD)为代表的自由基清除系统的影响发现当 Pb2+浓度高于 20mg·L-1时,钝顶螺旋藻生长受抑制,藻细胞随着浓度的升高和时间的延长而黄化死亡.细胞可溶性蛋白含量、藻蓝蛋白含量、超氧物歧化酶(SOD)活性均随着Pb2+浓度增加而逐渐下降.是极显著的负相关.低浓度Pb2+使光合速率大幅度下降但完整细胞的吸收光谱在高浓度时才发生急剧变化.Pb2+处理使超氧阴离子O2-和丙二醛(MDA)含量上升,其峰值分别出现在 30mg· L-1和 40mg· L-1处理组. SOD活性在 20mg· L-1以上显著下降,但其比活性在20-50mg·L-1范围内仍维持较高水平,且相对稳定. |
| 关键词: 铅污染 钝顶螺旋藻 生长量 超氧物歧化酶活性 |
| DOI：10.18307/1999.0208 |
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| 基金项目:江苏省自然科学基金资助项目(BJ98077) |
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| Effect of Pb2+ on the Growth of Spirulina platensis and Its Physiological Characters |
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ZHOU Chang-fang, WU Guo-rong, LU Chang-mei, CHEN Guo-xiang, WEI Jin-cheng
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Deportment of Biology Naming Normal Uniwrsity, Naming 210008
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| Abstract: |
| In this paper, we studied the growth of S. platensis in mimic Pb2+ polluted environment with different concentration from 10 to 80 mg·L-1. The results showed that the growth rate of S. platensis was inhibited by Pb2+ above 20mg·-1, S. platensis yellowed and died with Pb2+ concentration increasing and time going. The solvable protein, phycocynin and the ratio of phyco-cynin to carotenonid were all decreased. Each had a noticeable correlation with the Pb2+ concen-tration. Lower concentration of Pb2+ could decrease the photosyntheitc O2 evolution of S. plat-ensis, yet the absorption spectrum of S. platensis was only seriously changed on high Pb2+ con-centration. Contents of O2- and MDA were also changed, each reached its maximum at 30mg·L-1 and 40mg·L-1. The activity of SOD was decreased obviiusly when above 20mg·L -1, but its relative activity was higher and quite steady at Pb2+ concentration from 20 to 50mg.L-1. |
| Key words: Pb2+ pollution Spirulina platensis growth rate SOD activity |
