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引用本文:周易勇,夏宜琤.富营养水体磷酸酶动力学参数的季节变化——水体酶研究之一.湖泊科学,1993,5(4):335-340. DOI:10.18307/1993.0406
Zhou Yiyong,Xia Yicheng.Seasonal of alkaline variation of kinetics parameters phosphatase in the eutrophic zone of a shallow chinese freshwater lake (donghu lake). J. Lake Sci.1993,5(4):335-340. DOI:10.18307/1993.0406
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富营养水体磷酸酶动力学参数的季节变化——水体酶研究之一
周易勇, 夏宜琤
中国科学院水生生物研究所, 武汉 430072
摘要:
本文报道了武汉东湖富营养湖区(东湖Ⅰ站)水体磷酸酶动力学参数(最大反应速度Vmax和米氏常数Km)的季节变化规律, 在6月、8月和11月(或邻近时间), 湖水溶解正磷酸盐的浓度较低而叶绿素的浓度达到高峰值, 同时, Vmax达到最大值, Km值明显减小。因此, 浮游植物可以通过提高酶的反应速度以及增强酶与底物的亲和能力这两种方式有效地利用有机磷。实验结果表明, 湖水中对紫外线敏感的磷化合物可能是磷酸酶的竞争性抑制剂。
关键词:  水体磷酸酶  对紫外线敏感的磷化合物  湖泊磷循环
DOI:10.18307/1993.0406
分类号:
基金项目:淡水生态与生物技术开放实验室和本所所长择优基金
Seasonal of alkaline variation of kinetics parameters phosphatase in the eutrophic zone of a shallow chinese freshwater lake (donghu lake)
Zhou Yiyong, Xia Yicheng
Institute of Hydrobiology, Academia Sinica, Wuhan 430072
Abstract:
Seasonal variation of kinetics parameters of total alkaline phosphatase in the eutrophic zone of a shallow Chinese freshwater lake (Donghu Lake) was reported.The concentration of chlorophyll and Vmax of total alkaline phosphatase reached the highest level when ambient orthophosphate concentration was low and the values of Michaelis constant(Km)of alkaline phosphatase decreased.Thus, phytoplankton seems to compensate for their phosphorus deficiency not only by an increase in enzyme activity but also an improved ability to use low substrate concentrations. The km of alkaline phosphatase bore a direct positive relation to UV-sensitive P compounds (UVSP). A better relationship by plotting him values against the ratio UV SP:phosphmmonoester is also found, suggesting that UVSP is a competitive inhibitor of alkaline phosphatase activii.yin the lake.
Key words:  Phosphatase in aquatic environment  UV-sensitive P.compounds  P.cycling in lake
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