In the present study, we investigated spatial and temporal distributions of sulfate reducing bacteria (SRB) and controlling factors in sediment of Aha Reservoir, Lake Hongfeng and Lake Baihua, Guiyang City based on the most probable number (MPN) method. The results indicate that there were discernible seasonal variations in the SRB content distribution. SRB contents of sediments in winter were much less than those in summer and fall. The peaks of SRB contents occurred in the middle of sediment cores in winter, while SRB generally increased towards the sediment-water interface in June and September. The contents of sulfate in the sediments in Aha Reservoir which has been polluted by acid mine drainage were much higher than those in the other two reservoirs:Lake Hongfeng and Lake Baihua. SRB contents, however, have no discernible spatial variations in the three lakes(reservoirs), except for elevated values only in the surface of sediment in Lake Aha in summer and autumn. This indicates that higher organic matter contents and optimum pH in these reservoirs favor the growth of SRB, and sulfate content was not the main significant factor of SRB growth.