This paper deals with the results with regard to protozoan community diversity of Stechlin Lake in Berlin Germany, conducted during November 1994 and January, 1995. About 128 species of protozoa have been first recorded following the examination of colonization on polyurethane foam unit (PFU) artificial substrates and various types of natural substrates in this lake. The differences in species richness individual abundance and rates of PFU protozoan colonization at three sampling sites with the different habitat conditions are described. Based on analysis and comparison, PFU can collect more protozoan species than natural substrates do. The results of this research provide further evidence that the PFU method offers the advantages of sampling protozoa compared with other routine collecting methods.