Inland lakes potential emit large nitrous oxide (N₂O) to atmosphere and play significant role in global N₂O budget estimation. Widespread global increase in lake phytoplankton blooms have been found, identifying the N₂O flux and its influencing factors at the cyanobacteria-dominated lake is essential to being able to accurate estimate the N₂O emissions from lakes and predict future change. In order to identify N₂O flux characteristics and its influencing factors in the cyanobacteria-dominated zone of Lake Taihu, the N₂O emission was observed based on the two-year field measurements. Results showed the annual mean N₂O flux from the cyanobacteria-dominated zone ((4.88±3.05) mmol/(m²·d)) was about twice higher than that in the central zone with less cyanobacteria blooms ((2.10±4.31) mmol/(m²·d)). Meanwhile, the N₂O emissions varies spatially within the cyanobacteria-dominated zone, peak N₂O emission (10.93 mmol/(m²·d)) occurred in littoral zone due to the large external loadings input via river discharge. Continuous filed measurement showed that the N₂O emission varied seasonally, but the factors influencing the seasonal patterns varied among sub-zones. Generally, the temporal variation of N₂O emission were regulated by N loadings in littoral zone, and were regulated by multiple environment variables, such as temperature and N loadings, in Meiliang Bay and the central zone. High nitrogen loadings contributed to large N₂O emission from cyanobacteria-dominated zone, indicating that N loadings can be used as proxy of N₂O emission from lakes. Considering significant temporal and spatial variation of N₂O emission from cyanobacteria-dominated zones, more attention should be paid in future research.