In order to better understand the effect of cascade damming on river chemistry distribution in the karst region, we collected water samples from Hongjiadu Reservoir (multi-yearly regulation), Wujiangdu Reservoir (seasonal regulation) and Suofengying Reservoir (daily regulation) in January, April, July and October of 2017. We analyzed the water chemistry characteristics of the inflow, outflow and stratified water samples of these three reservoirs and investigated the cascade damming effect on water chemistry. The results show that water chemistry distribution has been highly affected by the damming effect. The HCO₃^- concentration of the hypolimnion is 12.9%, 5.5% and 8.0%, and the Ca²⁺ concentration is 15.9%, 2.4% and 8.5% higher than that of the surface water, respectively, for the Hongjiadu Reservoir, Suofengying Reservoir and Wujiangdu Reservoir. Consequently, the chemical weathering rate estimation of carbonate rock has also been affected. The annual weathering rate variation of Hongjiadu Reservoir, Suofengying Reservoir and Wujiangdu Reservoir is -1.7%-15.4%, -5.6%-1.1% and -0.3%-3.4%. The calculated weathering rate and HCO₃^- and Ca²⁺ concentrations in decreasing order is Hongjiadu Reservoir > Wujiangdu Reservoir > Suofengying Reservoir, which is consistent with the water retention time, indicating that water retention time affects the redistribution of water chemistry. At the same time, the ions concentration showed obvious seasonal variations, and the change rates in the three reservoirs were significantly greater during the flood season than that of the dry season. The above conclusions indicate that the water chemistry of rivers in karst area is highly affected by damming, which affects the estimation of chemical weathering rate. Water residence time and temperature are the main controlling factors on water chemistry redistribution. Therefore, we proposed that the damming effects should be taken into account to calculate the weathering rate accurately.