To explore the method of microchemistry marking on the otolith of Aristichthys nobilis,the 1-year-old juveniles were reared at five different concentrations(1, 2, 4, 8, 16 mg/L) for 28 days, and the exogenous Sr sedimentation in otoliths were detected with electron probe microanalyzer (EPMA). The mapping analysis showed that obvious strontium signatures in the edge of otoliths of juveniles were produced at different concentrations of Sr. The yellow-green strontium bandappeared in low level, the deep red zone shown as the concentration gradually increased. Line analysis indicated that the Sr/Ca ratio of control group was stable at 2.14±0.39, but which in the experimental groups of the different concentrations all appeared quite dramatically peak strontium value(5.04-32.32). Significant linear relationship was found between exogenous Sr and the Sr/Ca ratio in the edge of otoliths. There was also no significant difference in death rate between control and experimental groups. All of these suggested that the method of Sr marking confirmed the feasibility in recognizing the releasing individuals of A. nobilis. We suggest that stock discrimination of A. nobilis were marked in larval and juvenile and the concentration of exogenous Sr should be 4 mg/L, lasting for more than 28 days.