Lake Jiaogang is a natural lake on the left bank of the Huaihe River, which plays an important role in flood control, irrigation, aquaculture and tourism. In this study, spatio-temporal variation and impact parameters of the water quality were analyzed using the monitoring data of the four seasons of Lake Jiaogang, by using Kriging method. The result indicated that the water quality parameters of Lake Jiaogang showed changes at temporal and spatial scale due to the effects of hydrology and human activities. In views of seasonal changes, the water transparency was lower in summer and was higher in autumn. Dissolved oxygen concentration in spring was significantly higher than that in summer, autumn and winter. The concentrations of total nitrogen, total phosphorus and permanganate index were the highest in summer and the lowest in autumn. The spatial difference of transparency of the four seasons was significant. The spatial distribution of dissolved oxygen was more uniform in spring and winter. The summer dissolved oxygen showed a tendency that the concentration in the central lake was higher than the surroundings. The dissolved oxygen concentration in autumn was high in the west and low in the east of the lake. The distribution of total phosphorus was much uniform in spring, and the total phosphorus concentration was high in the west and low in the east in summer, autumn and winter. Index of permanganate concentration showed a tendency that it was high in the east and low in the west in spring and autumn, which a high index was mainly concentrated in the north lake in summer but the index changed little in winter.