This study aims at understanding the distribution characteristic and the correlativity of total nitrogen (TN),total phosphorus (TP) , hydrogen isotope(D)and oxygen isotope(¹⁸O)in ice layer and water during the freeze-up period in Lake Dalinuoer of Inner Mongolia. The results show that: 1)The Lake Dalinuoer water with high content of nutrient is even worse than the fifth level of water quality(Chinese standard) during freeze-up period. The concentrations of TN and TP in water are higher than those of in ice layer above the water during the freeze-up period. They are 9.91 times and 3.11 times in water higher than those of the ice layer, respectively. It is caused by the process of concentration effect on nitrogen and phosphorus when the lake water freezes up. It can be found that the interception content of nitrogen is relatively higher than phosphorus in the ice layer,comparing with the unfrozen period.2)The effect of thermodynamic isotope fractionation and the inflow supplies of Gongger River together make that isotope content in ice much larger in water during freeze-up period. The isotope content is increasing from the top ice to the bottom one. 3) The content of isotopic and nutrient appeares significantly negative correlation during the freeze-up period. The correlation relationship in water is stronger than that in ice, and bottom ice stronger than the top one. It was found that the closest correlation coefficients is 0.991 in lake water, and 0.988 in ice, calculated by SPSS software. The quantitative relationship between the content of isotope and nutrient (eg. the relationship function: TN=-0.2825δD-6.0083 and TP=-0.0805δD-1.2395) will provide a new method for studying temporal and spatial variation of nutrient content in the lake.