The effects of apical tip, node number and burial depth on regeneration abilities of vegetative fragments of six submerged plant species Myriophyllum spicatum, Hydrilla verticillata, Potamogeton pectinatus, P.crispus, Ceratophyllum demersum and Chara spp., were conducted.The results showed no significant changes occurred in the numbers of regenerated shoots and roots on fragments decapitated compared with the fragments with apices, except apical fragments of P.crspus had significanfly lower regeneration abilities.The increased numbers of nodes significantly enhanced the regeneration abilities of shoots and roots generally, and the fragments with above five nodes regenerated better than others.The fragment of M.spicatum and less than six-node of H.verticillata were able to regenerate new shoots and roots only at 0cm burial depth, while the other four species were able to regenerate new shoots and/or roots at depths of 1 cm and 2cm.Fragment regeneration abilities varied markedly both within and bctween the species.Both C.demersum and Chara spp.had the highest regeneration ability of shoots, but failed to regenerate roots.