The profundal community of macrozoobenthos in Lake Qiandao, Zhejiang Province, China has been seasonally investiga-ted from January 2007 to October 2008. A total of eight taxa were collected, among which 7 taxa belonged to oligochaeta and oneto nematoda. Limnodrilus hoffineisteri and Tubifex tubifex dominated the communities of macrozoobenthos, and type of which showedno seasonal vacation. Annual mean density and biomass were 594. hind./m² and 0.54g/m²,respectively, and they neither dis-play any seasonal variation. However, both differed significantly in horizontal distribution with the maximum at the upper reachesand the minimum at the lower reaches. Density and biomass both negatively significantly correlated with water depth and secchidepth. Density positively correlated with TN and TP of mixed water samples of water column(MWSWC) and with TP of local watersamples at bottom (LWSB),respectively, and biomass positively correlated with TP of MWSWC. Only water depth from eight mainphysical and chemical parameters of LWSB could be accepted finally by the stepwise regression models and used to explain the vac-anon of density and biomass, which decreased exponentially with water depth.