Transplantation of hydrophytes is an important conduct during the construction of constructed wetland. Transplantingstress could influence the survival ratio and growth of hydrophyte. The change of transplanting stress with time after lifting on Arua-do donax, Phragmites communis and Typha latifolia in lake Nansi, Shandong Province was studied in this paper. The results wereas follows; P. communis had the least decrease in biomass and chlorophyll content among the 3 hydrophytes, while the chlorophyllcontent of A. donax decreased rapidly after lifting. In teens of the chlorophyll fluorescence, A. donax had the largest latent activity(Fv/Fo) of Photosynthesis System Ⅱ(Φ_PSⅡ),latent maximum photosynthesis capacity(Y) and light protective capacity(NPQ).T. latifolia had the highest actual photosynthesis efficiency(Φ_PSⅡ)synchronously. Photochemical quenching, lightprotective capacity and actual photosynthesis efficiency of the 3 hydrophytes all decreased to the lowest in 5 hours after lifting.However, the latent maximum photosynthesis capacity reached the lowest after 22 hours. TheΦ_PSⅡ, Y, qP, NPQ of P. communis and Φ_PSⅡ,qP of T. latifolia showed remarkable positive correlation with the change of chlorophyll content (P<0.O1).The re-sups showed that the transplanting stress had great effects nn the 3 hydrophytes, and the degree of influence was following as P.communis>T.latifolia>A. donax, indicating the plants should be planted in 4-6h, 6-8h and 14-16h after lifting respective-1y, to sustain high survival ratio and natural growth.