It is an important part of harmonizing the spatial relationship between development and ecological protection anddevelopment leading and space control to adjust the spatial arrangement of industry and town according to the distribution ofecological protection space. Taking Jiangsu Province as a case, matrix-analysis and spatial analysis method was applied to exploringthe spatial coupling characteristic of ecological function protection area and construction land and status of the ecological functionarea occupied by space development based on extracting the information of ecological functional protection zones and constructionland distribution, later the adjusting route of space order was recommended. Conclusions were as follows: Firstly, the spatial couplingrelationship between ecological function protection area and construction land was harmonious in most part of Jiangsu Province, butSuzhou and Wuxi cities were out of line, the construction land’s expanding should be decreased in the future. Secondly, constructionland occupies a large part of important everglade, riverhead protection area, and landscape area, spatial development should keepaway from them. Thirdly, the ecological function area covered by the rural resident area is larger than other kind construction land, sothe pressure on the region’s ecosystem can be alleviated by the way of changing the spatial distribution of rural resident area in thefurther.