It is becoming an urgent problem in the lake environmental treatment that how to effectively remove the highly frequently cyanobacterial bloom. The purpose of the paper is to research the surfactant—Hexadecyl trimethyl ammonium bromide (CTAB)-mediated in-situ-sediment to remove the cyanobacterial through agitation effect and stimulating the water column disturbing through sediment resuspention generator in the laboratory. The results showed that the blue-green algal's removal efficiency could reach 83.9% after 30min water body still at the dosage adding of CTAB and sediment were (0.30+0.20)g/L; and the removal efficiency reached 98.9% after water body still. Preliminary results showed that the CTAB (0.30g/L dosage) could effectively remove the cyanobacterial in the lab, and also the turbidity of the water body had the corresponding change. The SEM images of the CTAB-flocculated algae showed that the netting and antivirus could be an important role in the alge cell flocculation. However, the antivirus role of CTAB could cause the risk that the cell-inclusion would release to the lake water.