Lake Taihu, which has been in serious eutrophic pollution status, was selected to be the study area. In Lake Taihu, two-time experiments of airborne hyperspectral remote sensing were carried out, covering seven airborne strips over Lake Taihu in both winter and summer. Besides of the two times of experiments, the in-situ inherent and apparent optical properties of Lake Taihu water were measured and analyzed for additional four times. The specific inherent optical property database of Lake Taihu was built. Based on the database and bio-optical model, analytical approaches were developed to retrieve chlorophyll, total suspended matter, and yellow substance. To validate these analytical approaches, airborne hyperspectral remote sensor WHI image and spaceborne hyperspectral remote sensor CHRIS image were used to retrieve water quality parameters, and the results were good